PCA-TTC显色法测定工业循环水中异养菌的实验研究  

Determination of Heterotrophic Bacteria in Industrial Circulating Water by PCA-TTC Chromogenic Method

作　　者：娄红杰 LOU Hongjie

机构地区：[1]国能榆林化工有限公司,陕西神木719300

出　　处：《科技创新与应用》2022年第31期48-53,共6页Technology Innovation and Application

基　　金：国家能源集团国能榆林化工有限公司科技创新项目(YLHG-2021-02)。

摘　　要：为便于菌落生长和计数,建立PCA-TTC显色法测定水中异养菌的分析方法。在标准平皿计数法基础上,用平板计数培养基(PCA)替代营养琼脂培养基(NA),并在PCA中加入2,3,5-氯化三苯基四氮唑(TTC),使菌落生长成红色。通过正交试验显色和计数结果,确定TTC在PCA中的最适浓度为0.002%(质量分数),培养时间为3 d。结果表明,在优化的试验条件下,对循环水水样测定,与标准NA平皿计数法对比,经卡方检验,P>0.05,2种方法虽无显著差异,但PCA-TTC显色法检出率高于NA平皿计数法,可以替代标准平皿计数法,且培养时间从72 h缩至66 h时异养菌总数可得到准确的结果。该方法不仅对受损细菌恢复并进行有效促进,易于辨别,有效消除颗料物和气泡干扰,还有助于降低TTC灭菌操作难度和抑菌性。In order to facilitate colony growth and enumeration, an analytical method for the determination of heterotrophic bacteria in water by PCA-TTC chromogenic method was established. On the basis of standard plate counting method, plate count agar(PCA) medium was used to replace nutrient agar(NA) medium, and 2,3,5-triphenyltetrazolium chloride(TTC) was added to PCA to make colonies grow red. The optimum concentration of TTC in PCA was determined to be 0.002%(mass fraction) through the results of color development and counting by orthogonal test, and the incubation time was 3 d. The research showed that, under the optimized test conditions, the determination of circulating water samples was compared with the standard NA plate counting method, and the chi-square test was performed, P>0.05. Although there was no significant difference between the two methods, the PCA-TTC colorimetric method detected The yield rate is higher than the NA plate counting method, which can replace the standard plate counting method, and the total number of heterotrophic bacteria can be obtained when the culture time is shortened from 72 h to 66 h. This method not only restores and effectively promotes damaged bacteria, is easy to identify, and effectively eliminates the interference of particles and air bubbles, but also reduces the difficulty of TTC sterilization and its bacteriostatic properties.

关 键 词：平板计数培养基(PCA) 营养琼脂培养基(NA) TTC显色法 循环水 异养菌 

分 类 号：X832[环境科学与工程—环境工程]