光散射光谱在生物医学领域的发展和应用  

Development and application of light scattering spectroscopy in biomedical field

作　　者：朱亮 董理 陈晋 李泽臻[3] 许嘉悦 严燕丽 邱俊 李晓天[4] ZHU Liang;DONG Li;CHEN Jin;LI Zezhen;XU Jiayue;YAN Yanli;QIU Jun;LI Xiaotian(School of Biomedical Engineering,Guangdong Medical University,Dongguan 523808,China;Guangdong University of Foreign Studies,Guangzhou 510420,China;Guangdong Provincial People's Hospital,Guangzhou 510080,China;Changchun Institute of Optics,Fine Mechanicsand Physics,Chinese Academy of Sciences,Changchun 130033,China)

机构地区：[1]广东医科大学生物医学工程学院,广东东莞523808 [2]广东外语外贸大学,广东广州510420 [3]广东省人民医院,广东广州510080 [4]中国科学院长春光学精密机械与物理研究所,吉林长春130033

出　　处：《光散射学报》2022年第2期103-115,共13页The Journal of Light Scattering

基　　金：国家自然科学基金(6150524);国家重大科学仪器设备开发专项项目(2014YQ1203510220190302047GX);广东省基础与应用基础研究基金联合基金(2019A1515110572)。

摘　　要：光散射光谱(Light Scattering Spectroscopy,LSS)是一种可以实时、无创地对细胞核以及细胞器结构进行探测的光谱技术,并且能够在细胞和亚细胞尺度上获得生物组织的结构特征。LSS将微小粒子的光散射特性与它们的大小、折射率和形状联系了起来。生物组织具有结构特异性,当被光照射时会产生不同角度和偏振特性的散射光,因此可以获得有关组织宏观和微观的结构信息。这项技术在相关领域得到了深入的研究,并被扩展到癌细胞的检测。本文首先对LSS技术的基本原理进行了介绍,然后详细阐述了该技术在早期癌症诊断中的研究和应用,最后,我们总结了LSS技术的优势并对其未来的发展进行了展望。Light Scattering Spectroscopy is a spectroscopic technique which relates the scattering spectral properties of tiny particles to their size,refractive index and shape.LSS allowing real-time,non-invasive probing of cell nuclei and organelle structures and enables obtain the characterization of biological tissue structures at the cellular and subcellular scales.When biological tissues illuminated by a light source,it can produce scattered light with different angular and polarization characteristics due to its’structural specificity,so the information about the macroscopic and microscopic structures of tissues can be obtained.This technique has been intensively studied in related fields and extended to the detection of cancer cells.First,we introduce the basic principles of LSS technology,then a detail description of the research and application of this technology in early cancer diagnosis is discussed.Finally,we summarize the advantages of LSS technology and outlooks its future development.

关 键 词：光散射 癌症 光学诊断技术 

分 类 号：Q63[生物学—生物物理学]