生物组织大深度定量光学成像  

Large-Depth Quantitative Optical Imaging of Biological Tissues

作　　者：文志博 刘开元 蒋慎益 何木斌 韩涛[1] 斯科[1] 李鹏[1] 刘智毅 钱骏[1] 丁志华[1] Wen Zhibo;Liu Kaiyuan;Jiang Shenyi;He Mubin;Han Tao;Si Ke;Li Peng;Liu Zhiyi;Qian Jun;Ding Zhihua(State Key Laboratory of Modern Optical Instrumentation,College of Optical Science and Engineering,Zhejiang University,Hangzhou 310027,Zhejiang,China)

机构地区：[1]浙江大学光电科学与工程学院现代光学仪器国家重点实验室,浙江杭州310027

出　　处：《光学学报》2022年第17期322-342,共21页Acta Optica Sinica

基　　金：国家重点研发计划(2017YFA0700501,2019YFE0113700);国家自然科学基金(62035011,11974310,31927801,61735016,62075189,61975172,61905214);浙江省自然科学基金(LR22F050007,LR19F050002,LR20F050001);浙江大学教育部脑与脑机融合前沿科学中心。

摘　　要：近年来,面向生物组织大深度光学成像的方法不断发展,其中包括光学相干层析、多光子成像和自适应光学等。介绍了浙江大学光电科学与工程学院近年来在生物组织大深度定量光学成像方面的一系列重要进展,包括光学相干层析结构与功能成像、基于三光子荧光显微的大深度脑血管成像和新型的畸变误差波前校正方法等,并进一步概述了如何对上述方法获取的光学图像实施定量表征以获取生物组织的生理与病理信息。In recent years,methods for large-depth optical imaging of biological tissues,such as optical coherence tomography,multi-photon imaging,and adaptive optics,have been developed continuously.This paper outlines a series of important advances in large-depth quantitative optical imaging of biological tissues achieved by the College of Optical Science and Engineering of Zhejiang University in recent years,including structural and functional optical coherence tomography,large-depth cerebral vascular imaging based on three-photon fluorescence microscopy,and new wavefront correction methods for distortion errors.It also summarizes the ways of quantitatively characterizing the optical images obtained by these methods to acquire physiological and pathological information about biological tissues.

关 键 词：生物技术 大深度光学成像 光学相干层析 多光子成像 自适应光学 定量表征 

分 类 号：O439[机械工程—光学工程]