不同紫花苜蓿品种光合生理特性差异及香豆素含量的变化  

Differences in Photosynthetic Physiological Characteristics and Changes in Coumarin Content of Different Alfalfa(Medicago sativa)Varieties

作　　者：武蓓 师尚礼[1] 张辉辉 张晓燕[1] 吴芳[1] 王文娟 何龙 刘旵旵 WU Bei;SHI Shang-li;ZHANG Hui-hui;ZHANG Xiao-yan;WU Fang;WANG Wen-juan;HE Long;LIU Chan-chan(College of Pratacultural Science,Gansu Agricultural University/Key Laboratory of Grassland Ecosystem of Ministry of Education/Sino-U.S.Centers for Grazing Land Ecosystem Sustainability,Lanzhou,Gansu Province 730070,China)

机构地区：[1]甘肃农业大学草业学院/草业生态系统教育部重点实验室/中-美草地畜牧业可持续发展研究中心,甘肃兰州730070

出　　处：《草地学报》2022年第10期2675-2684,共10页Acta Agrestia Sinica

基　　金：中国工程院战略研究与咨询项目(2021-DFZD-21-4);国家自然科学基金-地区科学基金项目(32160330)资助。

摘　　要：本研究采用营养液沙培法,以9个紫花苜蓿品种为试验材料,比较分析紫花苜蓿香豆素含量和光合生理差异,以及光合生理对香豆素含量的影响,进一步明确影响香豆素含量高低的内在因素。结果表明:香豆素含量范围是12.08(‘清水苜蓿’)~20.12 mg·g^(-1)(‘陇中苜蓿’)。香豆素含量与蒸腾速率呈显著负相关(r=-0.675,P<0.05),‘清水苜蓿’蒸腾速率、净光合速率、气孔导度、叶绿素含量和可溶性蛋白含量高于‘陇中苜蓿’,胞间CO_(2)浓度、可溶性糖含量、蔗糖含量和淀粉含量低于‘陇中苜蓿’。通径分析结果表明,可溶性糖、可溶性蛋白、蔗糖和叶绿素b是影响香豆素含量的主要光合生理指标,可溶性糖和可溶性蛋白通过直接作用影响香豆素含量,叶绿素b和蔗糖通过可溶性蛋白产生间接作用影响香豆素含量。蒸腾速率是香豆素含量的主要决定因子,叶绿素b是主要限定因子。To understand the intrinsic factors affecting the level of coumarin content,the differences of coumarin content and photosynthetic physiology of nine sand culture alfalfa varieties were compared and the effect of photosynthetic physiology on the coumarin content of alfalfa were studied in this study.The results showed that their coumarin contents ranged from 12.08 mg·g^(-1)(Medicago sativa cv.‘Qingshui’)to 20.12 mg·g^(-1)(Medicago sativa cv.‘Longzhong’).The correlation coefficient between coumarin content and transpiration rate was 0.675,which was significantly negatively correlated(P<0.05).The transpiration rate,net photosynthetic rate,stomatal conductance,and the contents of chlorophyll and soluble protein of‘Qingshui’were higher than those of‘Longzhong’.The intercellular CO_(2) concentration,and the contents of soluble sugar,sucrose and starch were lower than those of‘Longzhong’.The results of path analysis showed that soluble sugar,soluble protein,sucrose and chlorophyll b were the main photosynthetic physiological indexes affecting the coumarin content.Soluble sugar and soluble protein mainly affected the coumarin content through direct effect.Chlorophyll b and sucrose mainly affected the coumarin content through indirect effect of soluble protein.The main determinant and imiting factor of coumarin content were transpiration rate and chlorophyll b,respectively.

关 键 词：紫花苜蓿 光合生理 香豆素含量 品种差异 

分 类 号：S963.223.3[农业科学—水产养殖]