吉林省主推玉米品种的SSR分子标记纯度鉴定  

Purity Identification of SSR Molecular Markers for Main Maize Varieties in Jilin Province

作　　者：段梦冉 刘丰泽 葛建镕[1] 易红梅[1] 杨洪明[4] 高玉倩 岳鹏武 马文宇 班秀丽 王凤格[1] Duan Mengran;Liu Fengze;Ge Jianrong;Yi Hongmei;Yang Hongming;Gao Yuqian;Yue Pengwu;Ma Wenyu;Ban Xiuli;Wang Fengge(Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding,Maize Research Institute,Beijing Academy of Agriculture&Forestry Sciences,Beijing 100097,China;College ofAgriculture,Jilin Agriculture University,Changchun 130118,Jilin,China;National Agriculture Technology Extension Service Center,Beijing 100125,China;Seed Management Station of Jilin Province,Changchun 130022,Jilin,China)

机构地区：[1]北京市农林科学院玉米研究所玉米DNA指纹及分子育种北京市重点实验室,北京100097 [2]吉林农业大学农学院,吉林长春130118 [3]全国农业技术推广服务中心,北京100125 [4]吉林省种子管理总站,吉林长春130022

出　　处：《作物杂志》2022年第5期34-41,共8页Crops

基　　金：北京市农林科学院科技创新能力建设专项(KJCX20200305)。

摘　　要：针对吉林省主推玉米品种,构建了一套科学高效的纯度鉴定体系。以吉林省主推的50个玉米品种为研究材料,从GB/T 39914-2021公布的40对SSR引物中筛选出9对用于吉林省主推玉米品种的纯度鉴定。筛选出的9对引物杂合度在82%~100%,平均杂合度为91.5%,PIC值在0.44~0.75,平均PIC值为0.63。该组引物多态性高、稳定性强、分辨率高且非特异扩增片段少。将9重扩增体系进行优化,形成吉林省主推玉米品种纯度鉴定体系。用这9对引物对吉林省主推的6个品种进行纯度鉴定,可准确检测出品种的典型株、自交苗和异型株,共检测出10个自交苗和7个异型株,6份样品最高纯度为98%,最低纯度为96%,鉴定结果与田间鉴定结果相关性系数为0.766,相关性较高,结果可靠。A scientific and efficient purity identification system has been constructed for the main varieties of maize in Jilin province.A total of 50 maize varieties mainly promoted by Jilin province were used as materials.Nine pairs of primers were selected from 40 pairs of SSR primers of GB/T 39914-2021 for the purity identification of the main maize varieties in Jilin.The heterozygosity of the nine pairs of primers were between 82%and 100%,with an average heterozygosity of 91.5%,and the PIC values were between 0.44-0.75,with an average PIC value of 0.63.The set of primers had high polymorphism,strong stability,high resolution,and few non-specific amplification fragments.The nine-fold amplification system was optimized to form a variety purity identification system for main maize varieties in Jilin.The nine pairs of primers were used to identify the purity of the six main varieties in Jilin and could accurately detect the typical strains,inbred seedlings and heterotypic strains of the varieties.A total of ten inbred seedlings and seven heterotypic strains were detected.The highest purity the six samples was 98%,and the lowest purity was 96%.The correlation coefficient between the identification results and the field identification results was 0.766,the correlation between the identification results was high and reliable.

关 键 词：玉米 吉林省 纯度 SSR 

分 类 号：S513[农业科学—作物学]