向日葵抗盐碱生理生化机制与生长发育特性分析  

Analysis of Physiological and Biochemical Mechanism and Growth and Development Characteristics of Saline and Alkali Resistance in Sunflower

作　　者：贾秀苹[1] 卯旭辉[1] 梁根生[1] 刘润萍[2] 刘风[2] 王兴珍[1] Jia Xiuping;Mao Xuhui;Liang Gensheng;Liu Runping;Liu Feng;Wang Xingzhen(Institute of Crop,Gansu Academy of Agriculture Sciences,Lanzhou 730070,Gansu,China;Institute of Agricultural Economics and Information,Gansu Academy of Agricultural Sciences,Lanzhou 730070,Gansu,China)

机构地区：[1]甘肃省农业科学院作物研究所,甘肃兰州730070 [2]甘肃省农业科学院农业经济与信息研究所,甘肃兰州730070

出　　处：《作物杂志》2022年第5期146-152,共7页Crops

基　　金：国家自然科学基金(31360343);甘肃省科协学会(甘科协学20190002);国家特色油料产业技术体系建设项目(CARS-14-2-22);甘肃省特色作物产业技术体系。

摘　　要：以Y05-222A和Y06-136R杂交得到的135株F_(2)群体为研究材料,测定过氧化物酶(POD)、超氧化物歧化酶(SOD)、脯氨酸(Pro)、过氧化氢酶(CAT)、可溶性蛋白和丙二醛(MDA)等6个生理指标,并进行方差分析、相关性分析和通径分析,对F_(2)群体进行偏度及峰度分析。结果显示,以上6个生理指标在F_(2)群体中P>0.05,分布频率符合正态分布,同时存在双向超亲分离现象;相关性分析和通径分析显示,这些指标与抗盐碱系数均呈极显著相关(正相关或负相关),且相关系数与总间接通径系数方向一致。POD活性的直接通径系数为0.5003,可见POD直接影响抗盐碱性;CAT活性和Pro含量的直接通径系数分别为-0.1317和-0.0384,间接影响抗盐碱性;SOD活性、MDA及可溶性蛋白含量直接或间接影响抗盐碱性。POD、SOD、Pro、CAT、可溶性蛋白和MDA各生理指标的抗盐碱作用表现为POD>CAT>Pro>可溶性蛋白>MDA>SOD。叶片数、株高、茎粗和盘径与抗盐碱系数呈极显著正相关,其相关性表现为叶片数>盘径>株高>茎粗。以上结果可为研究油用向日葵抗盐碱性提供一定的理论基础。A total of 135 strains of the F_(2) population,produced by crossing of Y05-222A and Y06-136R,were used as materials to assess the levels of proline(Pro),soluble protein,malondialdehyde(MDA)as well as the activities of peroxidase(POD),superoxide dismutase(SOD)and catalase(CAT),and variance,correlation,and path analyses were performed.The F_(2) populationʼs skewness and kurtosis were also examined.The outcomes demonstrated that the frequencies followed a normal distribution and the six physiological indexes of the F_(2) population were P>0.05.It simultaneously demonstrated the separation of the bidirectional superaffinity.The six physiological indexes had highly correlations with salinity and alkali resistance coefficients,according to correlation and path analysis.The direction of the total indirect path coefficient and correlation was identical.The direct path coefficient of POD activity was 0.5003,which indicated it directly affected saline and alkali resistance.The direct path coefficients of CAT activity and Pro content were-0.1317 and-0.0384,respectively,which indicated it indirectly affected saline and alkali resistance,and SOD,MDA and soluble protein directly or indirectly affected salinity resistance.The Anti-saline effects of POD,SOD,Pro,CAT,soluble protein,MDA showed POD>CAT>Pro>soluble protein>MDA>SOD.Number of leaves,plant height,stem diameter and disk diameter had significant positive correlation with the salinity and alkali resistance coefficient.And the correlation showed number of leaves>disk diameter>plant height>stem diameter.The results provide theoretical basis for salinity salt and alkali resistance of oil sunflower.

关 键 词：油用向日葵 F_(2)群体 相关性分析 通径分析 

分 类 号：S565.5[农业科学—作物学]