高变八聚体寡核苷酸指纹基因分型法在石家庄地区布鲁氏菌分子流行病学研究中的应用  

Application of hypervariable octameric oligonucleotide finger-prints genotyping on molecular epidemiologic study of Brucellosis in Shijiazhuang

作　　者：张志[1] 高会霞[1] 田岳飏[1] 李砚峰[2] 郑立恒[3] 李雅楠 ZHANG Zhi;GAO Huixia;TIAN Yueyang;LI Yanfeng;ZHENG Liheng;LI Yanan(Department of Clinical Laboratory,the Fifth Hospital of Shijiazhuang,Hebei Province,Shijiazhuang050021,China;Department of Clinical Laboratory,Hebei Province Veterans Hospital,Hebei Province,Baoding071000,China;Department of Clinical Laboratory,Hebei Chest Hospital,Hebei Province,Shijiazhuang050000,China;Department of Clinical Diagnosis,Pingan Hospital,Hebei Province,Shijiazhuang050000,China)

机构地区：[1]石家庄市第五医院检验科,河北石家庄050021 [2]河北省荣军医院检验科,河北保定071000 [3]河北省胸科医院检验科,河北石家庄050000 [4]石家庄平安医院实验诊断学部,河北石家庄050000

出　　处：《中国医药导报》2022年第28期154-157,共4页China Medical Herald

基　　金：河北省医学科学研究重点课题(20191451)。

摘　　要：目的探索高变八聚体寡核苷酸指纹(HOOF-Prints)基因分型法在石家庄地区布鲁氏菌分子流行病学研究中的应用。方法选取2016年1月至2018年12月石家庄市第五医院收治的145例确诊布鲁氏菌病患者,经分离培养获得布鲁氏菌菌株。采用HOOF-Prints基因分型法进行分子流行病学研究。结果石家庄地区布鲁氏菌的流行生物种为羊种和牛种为主,羊种所占比例最大。羊3型和羊2型为主要流行菌株。结论石家庄地区布鲁氏菌的主要流行菌株为羊3型和羊2型。HOOF-Prints基因分型法是一种有效的布鲁氏菌分子流行病学研究方法。Objective To explore the application of hypervariable octameric oligonucleotide finger-prints(HOOF-Prints)genotyping on molecular epidemiologic study of Brucellosis in Shijiazhuang.Methods A total of 145 patients with confirmed Brucellosis admitted to the Fifth Hospital of Shijiazhuang from January 2016 to December 2018 were selected,Brucella strains were isolated and cultured.HOOF-Prints genotyping was used for molecular epidemiologic study.Results The most prevalent species of Brucella in Shijiazhuang were B.melitensis and B.abortus.Among them,B.melitensis biover 3 and B.melitensis biover 2 were the main epidemic strains.Conclusion The main epidemic strains of Brucella in Shijiazhuang are B.melitensis biover 3 and B.melitensis biover 2.HOOF-Prints genotyping is an effective method for the molecular epidemiologic study on Brucella.

关 键 词：布鲁氏菌病 布鲁氏菌 流行病学研究 多位点序列分型 

分 类 号：R516.7[医药卫生—内科学]