卡维地洛预处理的人脐静脉内皮细胞低密度脂蛋白诱导损伤情况观察  

Observation of low-density lipoprotein-induced injury in human umbilical vein endothelial cells pretreated with carvedilol

作　　者：徐红[1] 补娟[2] 闫凯欣 周玲[2] XU Hong;BU Juan;YAN Kaixin;ZHOU Ling(Geriatric Center,People's Hospital of Xinjiang Uygur Autonomous Region,Urumqi 830001,China)

机构地区：[1]新疆维吾尔自治区人民医院老年医学中心,乌鲁木齐830001 [2]新疆维吾尔自治区人民医院基础医学教育中心

出　　处：《山东医药》2022年第24期6-9,共4页Shandong Medical Journal

基　　金：新疆维吾尔自治区自然科学基金(2019D01C113)。

摘　　要：目的 观察卡维地洛预处理的人脐静脉内皮细胞(HUVECs)氧化性低密度脂蛋白(ox-LDL)诱导损伤情况。方法 体外培养HUVECs,将细胞分为5组,A、B、C组分别加入5、10、20μmol/L卡维地洛预处理6 h,再加入100μg/mL的ox-LDL诱导培养24 h;D组仅加入100μg/mL的ox-LDL诱导培养24 h;E组加入不含细胞的DMEM(高糖)培养基。采用微板法检测各组细胞LDH活性,CCK-8法测算细胞增殖抑制率,油红O染色观察细胞内脂滴形成情况。结果 与E组比较,B、C、D组LDH活性增强(P均<0.05);与D组比较,A、B、C组LDH活性减弱(P均<0.05)。与E组比较,C、D组OD值减小(P均<0.05);与D组比较,A、B、C组细胞OD值及增殖率增加(P均<0.05);与A组比较,B细胞OD值及增殖率减小(P均<0.05)。与E组比较,D组细胞内红色脂滴积累显著增加;与D组比较,A、B、C组细胞胞内脂滴形成明显减轻,其中以A组抑制脂滴积累效果最为明显。结论 卡维地洛预处理可以减轻ox-LDL诱导HUVECs损伤,尤以浓度为5μmol/L时效果最佳。Objective To observe the oxidative low-density lipoprotein(ox-LDL)-induced injury of human umbilical vein endothelial cells(HUVECs) pretreated with carvedilol.Methods HUVECs were cultured in vitro and divided into five groups.HUVECs in the groups A,B and C were treated with 5,10 and 20 μmol/L carvedilol for 6 h,and then100 μg/mL ox-LDL was added to culture for 24 h.HUVECs in the group D were incubated with 100 μg/mL ox-LDL for24 h.HUVECs in the group E were added with cell-free DMEM(high glucose) medium.The LDH activity of cells was detected by microplate method,the cell proliferation inhibition rate was measured by CCK-8,and the formation of intracellutar lipid droplets was observed by oil Red O staining.Results Compared with group E,LDH activity in the groups B,C and D significantly increased(all P<0.05).Compared with group D,LDH activity in the groups A,B and C decreased(all P<0.05).Compared with group E,OD values of groups C and D decreased(both P<0.05).Compared with group D,OD value and proliferation rate of cells in the groups A,B and C increased(all P<0.05).Compared with group A,the OD value and proliferation rate of cells in the group B decreased(both P<0.05).Compared with group E,the accumulation of red lipid droplets in the group D significantly increased.Compared with the group D,the formation of intracellular lipid droplets in the groups A,B and C was significantly reduced,and the effect of inhibiting lipid droplets accumulation in the group A was the most significant.Conclusion Carvedilol pretreatment can reduce ox-LDL-induced injury in HUVECs,especially at the concentration of 5 μmol/L.

关 键 词：动脉粥样硬化 卡维地洛 氧化性低密度脂蛋白 人脐静脉内皮细胞 

分 类 号：R543[医药卫生—心血管疾病]