中高温油藏内源微生物好氧激活规律  

Aerobic Stimulation Rules of Indigenous Microorganisms in High Temperature Reservoirs

作　　者：刘美辰 宋永亭 韩欢 高光军 李国强[1] 马挺[1] Liu Meichen;Song Yongting;Han Huan;Gao Guangjun;Li Guoqiang;Ma Ting(Key laboratory of Molecular Microbiology and Technology,Ministry of Education,College of Life Sciences,Nankai University,Tianjin 300071,China;Shengli Oilfield Petroleum Engineering Technology Research Institute,Dongying 257000,China)

机构地区：[1]南开大学生命科学学院,分子微生物学与技术教育部重点实验室,天津300071 [2]胜利油田石油工程技术研究院,山东东营257000

出　　处：《南开大学学报（自然科学版）》2022年第4期43-49,共7页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家863重点项目(2013AA064401);天津应用基础研究面上项目(16JCYBJC23700)。

摘　　要：针对胜利油田中高温油藏采出液,结合不同营养的激活实验和Miseq高通量测序技术,系统的研究了不同营养激活剂对中高温油藏内源微生物菌群变化的影响.结果表明,不同的营养物质均可激活油藏内源微生物,但在好氧条件下,只有特定的营养配方可以激活烃氧化菌.原油乳化效果的好坏与激活后样品中Bacilli纲内功能性微生物如Bacillus,Paenibacillus,Brevibacillus,Geobacillus是否被大量激活有相关性,以一定比例的糖蜜和玉米浆干粉作为激活剂可以有效激活上述功能微生物.The aerobic stimulation of indigenous microorganisms in high temperature reservoirs of Shengli Oilfield will combine with shake flask experiment and Miseq sequencing technologies.The results show that only the special activation system can be directional activate the hydrocarbon oxidizing bacteria we need.According to the Miseq sequencing analysis,crude oil emulsification degree is relative with the activation of Bacilli,especially the fuctional aerobic bacteria such as Bacillus,Paenibacillus,Brevibacillus,Geobacillus and so on.Molasses and corn syrup powder can effectively activate these fuctional bacteria.

关 键 词：中高温油藏 石油 生物乳化剂 内源微生物 微生物提高原油采收率 

分 类 号：Q939.99[生物学—微生物学]