补阳还五汤对多因素诱导急性血瘀证模型大鼠血小板功能及相关炎性因子的影响  

Effect of Buyang Huanwutang on Platelet Function and Inflammatory Cytokine in Rat Model of Acute Blood Stasis Induced by Multiple Factors

作　　者：王紫艳 李磊[1] 刘建勋[1] 孟红旭[1] 史跃[1] 马彦雷[1] 王奥奥 尤越 WANG Ziyan;LI Lei;LIU Jianxun;MENG Hongxu;SHI Yue;MA Yanlei;WANG Aoao;YOU Yue(Institute of Basic Medical Sciences of Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing Key Laboratory of Chinese Materia Pharmacology,Beijing 100091,China)

机构地区：[1]中国中医科学院西苑医院基础医学研究所国家中医心血管疾病临床医学研究中心中药药理北京市重点实验室,北京100091

出　　处：《中国实验方剂学杂志》2022年第21期1-9,共9页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然基金重点项目(82030124);国家中医药管理局中医药传承与创新“百千万”人才工程(岐黄工程)岐黄学者项目;中国中医科学院科技创新工程项目(CI2021A04609)。

摘　　要：目的:探究补阳还五汤对急性血瘀证模型大鼠的血小板功能及炎性因子的影响。方法:运用冰水浴复合注射肾上腺素的方法建立SD大鼠急性血瘀证模型,大鼠随机分为4组,分别为正常组、模型组、补阳还五汤组(3.2 g·kg^(-1))、阿司匹林组(60 mg·kg^(-1))。造模同时持续给药7 d,第8天皮下注射盐酸肾上腺素。观察中医证候宏观评价指标舌象、脉象,检测血液流变学、大鼠凝血功能、血小板聚集率等西医凝血系统评价指标,酶联免疫吸附测定法检测血清炎性及黏附因子相关指标[细胞间黏附分子-1(ICAM-1)、基质金属蛋白酶-9(MMP-9)]。结果:与正常组比较,模型组大鼠脉搏幅度显著降低(P<0.01),补阳还五汤较模型组可显著改善血瘀证大鼠脉象(P<0.01),模型组舌象呈现紫暗的血瘀证特点,舌底脉络瘀紫,舌面红(R)、绿(G)、蓝(B)值较正常组显著降低(P<0.01);与模型组比较,补阳还五汤可显著改善血瘀证大鼠舌象R、G、B值(P<0.01);与正常组比较,模型组血液黏度在高、中、低切剪应力下及血浆黏度检测值均明显升高(P<0.05,P<0.01),与模型组比较,补阳还五汤可以明显改善高、中、低切剪应力下的全血黏度及血浆黏度(P<0.05,P<0.01);凝血四项结果显示,模型组较正常组凝血酶原时间(PT)和凝血酶时间(TT)明显缩短(P<0.05,P<0.01),纤维蛋白原(FIB)含量显著升高(P<0.01);与模型组比较,补阳还五汤可显著改善血瘀证大鼠TT、FIB指标(P<0.01);模型组大鼠血小板聚集率(花生四烯酸、二磷酸腺苷诱导)也显著高于正常组大鼠(P<0.01),与模型组比较,补阳还五汤可显著降低血瘀证大鼠血小板聚集率(P<0.01);扫描电镜结果显示,与正常组比较,模型组血小板过度活化,伪足伸出明显,血小板之间聚集现象增多;与模型组比较,补阳还五汤组少见血小板活化和聚集现象。与正常组比较,模型组大鼠血清中炎性因子MMP-9和黏附因子ICAM-1含�Objective:To explore the effect of Buyang Huanwutang(BYHWT)on platelet function and inflammatory cytokines in the rat model of acute blood stasis.Method:The model of acute blood stasis was established with SD rats by ice water bath combined with injection of epinephrine.Rats were randomly assigned into four groups:normal group,model group,BYHWT(3.2 g·kg^(-1))group,and aspirin(60 mg·kg^(-1))group.The rats were injected with epinephrine hydrochloride on day 8 after 7 days of modeling.The macroscopic indexes of triditional Chinese medicine(TCM) syndrome including tongue manifestation and pulse manifestation were observed,while hemorheological indexes,blood coagulation,and platelet aggregation were detected.The serum levels of the inflammatory cytokine matrix metalloprotein-9(MMP-9)and the adhesion factor intercellular adhesion molecule-1(ICAM-1)and were determined by enzyme-linked immunosorbent assay(ELISA).Result:The pulse distention of rats in the model group was lower than that in the normal group(P<0.01),while BYHWT improved the pulse distention of the rats with the syndrome of blood stasis(P<0.01).In the model group,the tongue showed the characteristics of blood stasis syndrome,with dark purple veins at the tongue bottom and lower values of R,G,B on the tongue surface than those in the normal group(P<0.01),which,however,can be recovered by BYHWT(P<0.01).The blood viscosity at high,medium,and low shear stress and the plasma viscosity in the model group were higher than those in the normal group(P<0.01,P<0.05).Compared with the model group,BYHWT restored the whole blood viscosity under high,medium and low shear stress and plasma viscosity(P<0.05,P<0.01).The model group had shorter prothrombin time(PT),shorter thrombin time(TT),and higher fibrinogen(FIB)than the normal group(P<0.05,P<0.01).BYHWT improved the TT and reduced the FIB in the rats with blood stasis syndrome(P<0.01).The platelet aggregation rate induced by arachidonic acid(AA)and adenosine diphosphate(ADP)in the model group was higher than that in nor

关 键 词：血瘀证 证候 补阳还五汤 血小板 活化功能 炎性因子 

分 类 号：R2-0[医药卫生—中医学] R22R285.5R289R33