刺五加果实发育过程的代谢差异  

Metabolic Changes of Acanthopanax senticosus Fruits During Development

作　　者：孙菲阳 郭晓瑞[1] 宋莹[1] 徐明远 张晶[3] 唐中华[1] 倪红伟 SUN Feiyang;GUO Xiaorui;SONG Ying;XU Mingyuan;ZHANG Jing;TANG Zhonghua;NI Hongwei(College of Chemistry,Chemical Engineering and Resource Utilization,Northeast Forestry University,Harbin 150040,China;Heilongjiang Academy of Forestry,Harbin 150040,China;School of Clinical Medicine,Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]东北林业大学化学化工与资源利用学院,哈尔滨150040 [2]黑龙江省林业科学院,哈尔滨150040 [3]黑龙江中医药大学临床医学院,哈尔滨150040

出　　处：《中国实验方剂学杂志》2022年第21期163-170,共8页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技基础资源调查专项(2019FY100502);国家林业局林业科学技术推广项目([2019]12);黑龙江省博士后基金项目(LBH-Z20091);黑龙江省中医药科研课题项目(ZHY2022-138);黑龙江省中医药经典普及化专项课题项目(ZYW2022-059);黑龙江省自然科学基金联合引导项目(LH2022H084)。

摘　　要：目的:研究不同发育阶段的刺五加果实中初生代谢产物及酚类次生代谢产物的变化规律,为刺五加果实资源的合理利用提供理论基础。方法:基于气相色谱与质谱联用(GC-MS)技术与液相色谱与质谱联用(LC-MS)技术测定不同发育阶段刺五加果实中的初生代谢产物与酚类次生代谢物,利用多元统计分析的方法比较不同发育阶段刺五加果实的代谢差异。结果:利用GC-MS非靶向代谢组学技术在不同发育阶段的刺五加果实中共扫描到274个色谱峰,通过多元统计分析筛选鉴定得到24个初生差异代谢物,差异代谢物主要富集于磷酸戊糖途径、半乳糖代谢以及抗坏血酸和醛糖代谢途径。其中,磷酸戊糖途径、半乳糖代谢在转色后被激活,糖类在此时累积较多。抗坏血酸和醛糖代谢途径在转色前较为活跃,其终产物抗坏血酸在此时有较高的累积。利用超高液相色谱质谱联用技术(UPLC-MS)靶向测定了不同发育阶段果实中的28个酚类物质,其中,黄酮类主要在转色前的绿熟期累积,酚酸类主要在转色后累积。结论:不同发育阶段的刺五加果实中初生代谢物及酚类的累积显著不同。Objective:To study the changes of primary metabolites and phenols in the fruits of Acanthopanax senticosus at different development stages,so as to provide a theoretical basis for the rational utilization of A.senticosus fruit resources.Method:The primary metabolites and phenols in the fruits at different development stages were determined via gas chromatography-mass spectrometry(GC-MS) and liquid chromatography-mass spectrometry(LC-MS) and then compared by multivariate statistical analysis.Result:A total of 274 chromatographic peaks were obtained by GC-MS-based non-targeted metabonomics and 24differential metabolites were screened out by multivariate statistical analysis.The differential metabolites were mainly concentrated in pentose phosphate pathway,galactose metabolism,ascorbic acid and aldose metabolism pathways.After color conversion,the pentose phosphate pathway and galactose metabolism were activated and increasing sugars were accumulated.The ascorbic acid and aldose metabolism pathways were active before color conversion,with high accumulation of the end product ascorbic acid.The ultra-high liquid chromatography-mass spectrometry(UPLC-MS) identified 28 phenols in the fruits at different development stages.Flavonoids were accumulated mainly at the green ripening stage before color conversion,and phenolic acids were accumulated mainly after color conversion.Conclusion:The accumulation of primary metabolites and phenols in A.senticosus fruits varies significantly among different development stages.

关 键 词：刺五加果 代谢组学 多元统计分析 气相色谱-质谱联用 液相色谱-质谱联用 

分 类 号：R284.2[医药卫生—中药学] R289[医药卫生—中医学] R22R2-031R33