响应面结合遗传算法优化鹰嘴豆中抗炎活性成分的提取  

Optimization of Extraction of Anti-Inflammatory Components from Cicer arietinum L.by Response Surface Combined with Genetic Algorithm

作　　者：牛华周 康贺磊 侯万超 刘春明[1] 李赛男[1] 张语迟[1] 刘震 Niu Huazhou;Kang Helei;Hou Wanchao;Liu Chunming;Li Sainan;Zhang Yuchi;Liu Zhen(Central Laboratory,Changchun Normal University ,Changchun 130032;School of Mathematics,Changchun Normal University ,Changchun 130032)

机构地区：[1]长春师范大学中心实验室,长春130032 [2]长春师范大学数学学院,长春130032

出　　处：《中国粮油学报》2022年第9期132-137,共6页Journal of the Chinese Cereals and Oils Association

基　　金：国家自然科学基金(31870336);吉林省科技发展计划项目(20200201114JC);长师大自科合字(2020)第011号;长春师范大学研究生科研创新项目[2020]第060号。

摘　　要：以异黄酮提取率为评价指标,考察超声时间、乙醇浓度和料液比对鹰嘴豆异黄酮提取率的影响,在单因素实验基础上,利用响应面结合遗传算法优化鹰嘴豆异黄酮提取工艺,得到最佳提取工艺条件为:提取时间为1.73 h、乙醇浓度为78.64%,料液比1∶24.9 g/mL,鹰嘴豆中异黄酮提取率为0.76 mg/g。以5-脂氧合酶为作用靶点,利用超滤-质谱技术(UF-MS)筛选鹰嘴豆中具有抑制5-脂氧合酶作用的潜在抗炎活性成分。结果表明,利用响应面结合遗传算法可以进一步优化提高鹰嘴豆中活性成分的提取率;鹰嘴豆中具有潜在的抗炎活性成分为大豆苷、大豆苷元、毛蕊异黄酮、后莫紫檀素和鹰嘴豆芽素A,其结合强度分别为40.82%、36.93%、31.65%、28.75%和22.51%。The effects of ultrasonic-assisted ultrasonic time,concentrations of ethanol and ratio for material and liquid on the extraction yield of isoflavones were investigated for optimizing the ultrasonic-assisted extraction process of isoflavones from cicer arietinum L.On the basis of single factors,the best extraction process of isoflavones in Cicer arietinum L.was obtained by using the response surface combined with genetic algorithm:the extraction time of 1.73 h,the ethanol concentration of 78.64%and the liquid ratio of 1∶24.9 g/mL,and the extraction rate of isoflavones in cicer arietinum L.of 0.76 mg/g.As the 5-lipoxygenase was the target,the potential anti-inflammatory components with 5-lipoxygenase inhibition in cicer arietinum L.were screened by ultrafiltration mass spectrometry(UF-MS).The results indicated that the extraction rate of active ingredients in cicer arietinum L.could be further optimized by using the response surface binding genetic algorithm,and the potential anti-inflammatory active ingredients in cicer arietinum L.were daidzin,daidzein,calycosin,homoferreinrin and biochanin A,with degree of binding of 40.82%,36.93%,31.65%,28.75%and 22.51%,respectively.

关 键 词：鹰嘴豆(Cicer arietinum L.) 响应面(RSM) 遗传算法(GA) 超滤-质谱(UF-MS) 5-脂氧合酶(5-LOX) 

分 类 号：TQ646[化学工程—精细化工]