川产姜黄HPLC指纹图谱的研究及姜黄素类成分的测定  

Study on HPLC fingerprint and determination of curcuminoid in turmeric from Sichuan province

作　　者：刘芳[1] 华桦[2] 刘俐 张宇佳 王曙[1] 赵军宁 LIU Fang;HUA Hua;LIU Li;ZHANG Yujia;WANG Shu;ZHAO Juningn(West China School of Pharmacy,Sichuan University,Chengdu,Sichuan,610041 P.R.China;Sichuan Institute for Translational Chinese Medicine,Biological Assay Key Laboratory of State Administration of Traditional Chinese Medicine for Traditional Chinese Medicine Quality,Engineering Research Center for Formation Principle and Quality Evaluation of Genuine Medicinal Materials in Sichuan Province,Sichuan Engineering Technology Research Center of Genuine Regional Drug,Translational Chinese Medicine Key Laboratory of Sichuan Province,Institute of Translational Pharmacology and Clinical Application in Sichuan Academy of Chinese Medicine Sciences,Chengdu,Sichuan,610041 P.R.China;College of Food and Bioengineering,Chengdu University,Chengdu,Sichuan,610106P.R.China)

机构地区：[1]四川大学华西药学院,四川成都610041 [2]四川省中医药科学院转化药理与临床应用研究所,四川省中医药转化医学中心,国家中医药管理局中药质量生物评价重点研究室,四川省道地药材形成原理与品质评价工程研究中心,四川省道地药材系统开发工程技术研究中心,中医药转化医学四川省重点实验室,四川成都610041 [3]成都大学食品与生物工程学院,四川成都610106

出　　处：《华西药学杂志》2022年第5期541-544,共4页West China Journal of Pharmaceutical Sciences

基　　金：四川省科技厅重点研发项目(2018SZ0056);四川省中医药科技产业创新团队专项(2022C009)。

摘　　要：目的 评价川产姜黄的质量。方法 采用指纹图谱结合姜黄素类成分含量测定的方法评价川产姜黄的品质。结果 对照指纹图谱共标定了11个共有峰,样品与对照图谱的相似度均大于0.9。50批川产姜黄药材中姜黄素、去甲氧基姜黄素和双去甲氧基姜黄素的含量分别为0.93%~3.51%、0.31%~1.24%、0.07%~0.77%。犍为、沐川、宜宾产姜黄样品中姜黄素的含量均值明显高于药典标准。结论 所建指纹图谱可定性、定量评价川产姜黄的质量。OBJECTIVE To evaluate the quality of turmeric produced in Sichuan.METHODS Based on the fingerprint combined with content determination of curcuminoid to evaluate the quality of turmeric produced in Sichuan.RESULTS A total of 11 common peaks were calibrated in the fingerprint.The similarity between each sample and the fingerprint was greater than 0.9.The results showed that the contents of curcumin, demethoxylcurcumin and bisdemethoxycurcumin of turmeric in Sichuan province were 0.93%-3.51%,0.31%-1.24% and 0.07%-0.77%,respectively, and the mean content of curcumin in turmeric from Muchuan, Yibin, and Qianwei were obviously higher than the standard of pharmacopoeia.CONCLUSION The fingerprint can be used to evaluate the quality of Sichuan turmeric from qualitative and quantitative perspectives.

关 键 词：川产姜黄 高效液相色谱法 指纹图谱 姜黄素 去甲氧基姜黄素 双去甲氧基姜黄素 含量测定 品质评价 

分 类 号：R917[医药卫生—药物分析学]