HPLC同时测定抗宫炎片中的6种成分  

Simultaneous determination of six constituents in Kanggongyan tablets by HPLC

作　　者：万莉 牟腊梅 冉丽娟 阳文武 WAN Li;MU Lamei;RAN Lijuan;YANG Wenwu(Chongqing Key Laboratory of Development and Utilization of Genuine Medicinal Materials in Three Gorges Reservoir Area,Wanzhou Institute for Food and Drug Control,Chongqing,404000 P.R.China)

机构地区：[1]重庆市万州食品药品检验所,三峡库区道地药材开发利用重庆市重点实验室,重庆404000

出　　处：《华西药学杂志》2022年第5期545-548,共4页West China Journal of Pharmaceutical Sciences

基　　金：重庆市药品监督管局、重庆市科学技术局联合项目(渝药监[2020]34号)。

摘　　要：目的 采用HPLC法同时检测抗宫炎片中去甲异波尔定、盐酸益母草碱、连翘酯苷B、毛蕊花糖苷、金石蚕苷、异毛蕊花糖苷的含量。方法 采用资生堂反向C柱,以乙腈-0.5%磷酸溶液为流动相,梯度洗脱,检测波长依次为280 nm(去甲异波尔定)、218 nm(盐酸益母草碱)、332 nm(连翘酯苷B、毛蕊花糖苷、金石蚕苷、异毛蕊花糖苷),流速1.0 mL·min。结果 去甲异波尔定、盐酸益母草碱、连翘酯苷B、毛蕊花糖苷、金石蚕苷、异毛蕊花糖苷的线性范围分别为4.69~93.79、1.06~21.21、16.04~320.9、4.79~95.79、18.78~375.6、2.05~40.97μg·mL(r均≥0.9995),平均加样回收率为98.76%~102.00%,RSD为1.32%~1.73%。结论 所用方法操作简便、结果准确,可用于抗宫炎片中6种成分的含量测定。OBJECTIVE To establish an HPLC method for simultaneous determination of norisoboldine, leonurine hydrochloride, forsythoside B,verbascoside, poliumoside and isoacteoside in Kanggongyan tablets.METHODS The determination was performed on CAPCELL PAK C18 column with mobile phase consisted of acetonitrile-0.5 phosphoric acid solution(gradient elution).The detection wavelengths were 280 nm(norisoporidine),218 nm(leonuri hydrochloride) and 332 nm(forsythione B,verbascoside, poliumoside and isoacteoside),respectively.The flow speed was 1.0 mL·min.RESULTS Norisoboldine, leonurine hydrochloride, forsythoside B,verbascoside, poliumoside and isoacteoside showed good linear relationships within the ranges of 4.69-93.79,1.06-21.21,16.04-320.9,4.79-95.79,18.78-375.6,and 2.05-40.97 μg·mL(r≥0.9995),respectively.The average recoveries were 98.76%-102.00%,with the RSDs of 1.32%-1.73%.CONCLUSION The method is simple and accurate, which can be used for the determination of six components in Kanggongyan tablets.

关 键 词：抗宫炎片 高效液相色谱法 去甲异波尔定 盐酸益母草碱 连翘酯苷B 毛蕊花糖苷 金石蚕苷 异毛蕊花糖苷 含量测定 

分 类 号：R917[医药卫生—药物分析学]