基于框架核酸的细胞表面受体配体相互作用研究进展  

Advances in Receptor‑ligand Interactions on Cell Surface Based on Framework Nucleic Acids

作　　者：郭琳洁[1,2,3] 彭红珍 李江 王丽华[1,2,3] 诸颖 GUO Lin-Jie;PENG Hong-Zhen;LI Jiang;WANG Li-Hua;ZHU Ying(Division of Physical Biology,CAS Key Laboratory of Interfacial Physics and Technology,Shanghai Institute of Applied Physics,Chinese Academy of Sciences,Shanghai 201800,China;The Interdisciplinary Research Center,Shanghai Advanced Research Institute,Chinese Academy of Sciences,Shanghai 201210,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院上海应用物理研究所,中国科学院微观界面与探测重点实验室,上海201800 [2]中国科学院上海高等研究院,基础交叉研究中心,上海201210 [3]中国科学院大学,北京100049

出　　处：《应用化学》2022年第10期1475-1487,共13页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金(Nos.82050005,22022410);中国科学院青年创新促进会人才专项(No.2016236);上海市科委项目(No.19142202400)资助。

摘　　要：细胞表面受体与配体之间的特异性相互作用在细胞生物学过程中起着重要作用。然而,与均相溶液不同,受体分子在细胞膜上的分布是非连续的、动态的,因此细胞表面的受体配体相互作用通常呈现复杂的非线性结合模式。框架核酸作为一类具有确定几何形状的DNA纳米支架,可用于多价配体的偶联,为深入揭示受体配体相互作用机制提供了可靠的工具。利用框架核酸纳米分辨率的可寻址特性,可实现对配体数目、间距及空间构象等参数的精确调控,进而研究细胞表面受体配体的结合特性及影响因素,优化结合条件最终实现高效的分子识别及靶向治疗。本文综述了基于框架核酸的细胞表面受体配体相互作用研究进展,通过探讨细胞表面受体配体相互作用的重要影响因素及生物学应用,对该研究领域的发展前景和未来趋势予以展望。The specific interaction between receptor and ligand on cell surface plays an important role in cell biology.However,the distribution of receptor molecules on the cell membrane is discontinuous and dynamically different from that in the homogeneous solutions.The receptor-ligand interactions on the cell surface usually exhibit complex nonlinear binding patterns.In order to reveal the mechanisms,framework nucleic acids,as a class of DNA nanostructures with definite geometric shapes,could be used for the coupling of multivalent ligands.With the addressability at nanoscale,the number,spacing and spatial conformation of ligands are precisely regulated on framework nucleic acids.The binding characteristics between receptor and ligand on cell surface are studied,and efficient molecular recognition and targeted therapy could be achieved by optimizing these influencing factors.In this paper,the research progress of receptor-ligand interactions on cell surface based on framework nucleic acids is reviewed.By discussing the important influencing factors and biological applications,the future development of receptor-ligand interactions on cell surface based on framework nucleic acids is prospected.

关 键 词：框架核酸 受体配体相互作用 分子识别 靶向治疗 

分 类 号：O654[理学—分析化学]