杜仲F_(1)代叶表型变异分析及单株评价  

Analysis of Leaf Phenotypic Variation and Evaluation of Elite Individual Plant in F_(1) Progeny of Eucommia ulmoides

作　　者：庆军 孟益德 刘辰露 杜庆鑫[1,2,3] 杜红岩[1,2,3] 杜兰英[1,2,3] 黄海燕 李相军 王璐[1,2,3] Qing Jun;Meng Yide;Liu Chenlu;Du Qingxin;Du Hongyan;Du Lanying;Huang Haiyan;Li Xiangjun;Wang Lu(Research Institute of Non-timber Forestry,Chinese Academy of Forestry,Zhengzhou Henan 450003,China;Key Laboratory of National Forestry and Grassland Administraion on Non-timber Forest Germplasm Enhancement&Utilization,Research Institute of Non-timber Forestry,Chinese Academy of Forestry,Zhengzhou Henan 450003,China;Non-timber Forest R&D Center,Chinese Academy of Forestry,Zhengzhou Henan 450003,China;Central South University of Forestry and Technology,Changsha Hunan 410000,China;Henan Runyou Forestry Technical Service Co.Ltd.,Zhengzhou Henan 450008,China;Forestry Bureau of Hanbin District,Ankang Shaanxi 725000,China)

机构地区：[1]中国林业科学研究院经济林研究所,河南郑州450003 [2]中国林业科学研究院经济林研究所经济林种质创新与利用国家林业局重点实验室,河南郑州450003 [3]中国林业科学研究院经济林研究开发中心,河南郑州450003 [4]中南林业科技大,湖南长沙410000 [5]河南润友林业技术服务有限公司,河南郑州450008 [6]汉滨区林业局,陕西安康725000

出　　处：《西南林业大学学报（自然科学）》2022年第5期18-29,共12页Journal of Southwest Forestry University：Natural Sciences

基　　金：杜仲良种高效栽培与全质化利用示范(2017YFD0601302)资助。

摘　　要：通过离散描述、相关性分析、t检验及杂种优势等方法对“华仲8号”(♀)和“华仲12号”(♂)的109个杂交F_(1)代的17个叶表型性状进行遗传多样性分析,结合隶属函数分析筛选出叶表型性状连年稳定的F_(1)单株。结果表明:连续3年(2017—2019年)的杜仲F_(1)代叶片表型性状变异范围为1.55%~48.20%,叶片活性成分变异系数相对叶片形态变异系数较高、杜仲叶片活性成分具有更丰富的变异。桃叶珊瑚苷、京尼平苷酸、车叶草甘和绿原酸至少有1 a中2个表型性状呈极显著正相关,剩余12个叶形态性状中,叶面积、叶形指数和叶宽呈极显著负相关,叶长、叶宽和叶形指数呈极显著正相关。叶片红色成分、蓝色成分、绿色成分和叶绿素参考值的相关性达到极显著或显著水平,其中叶绿素参考值、叶片红色成分和绿色成分相关性均为极显著负相关。杜仲F_(1)代叶片部分性状之间相互制约,部分性状具有相对较为稳定的关系,但是整体性状之间的相关性关系较为复杂。最终获得叶表型连年稳定单株29个,其中最为稳定的为H8−8、H8−63和H8−66单株。The genetic diversity of 17 leaf phenotype in F_(1) progenies from the cross of'Huazhong No.8'and'Huazhong No.12'were analyzed by means of discrete description,correlation analysis,t-test and heterosis.And the F_(1) offspring with stable leaf phenotypic traits were screened by membership function analysis.The results showed that the variation of leaf phenotypes in F_(1) generation of Eucommia ulmoides from 2017 to 2019 ranged from 1.55%to 48.20%,and the variation coefficient of leaf active components was lower than leaf morphology,while leaf morphology had more variation.There was a very significant positive correlation between the 2 phenotypes of aucubin,genipinic acid,cypermethrin and chlorogenic acid in at least 1 year.Among the remaining 12 leaf morphological characters,there was a very significant negative correlation among leaf area,leaf shape index and leaf width,and an extremely significant positive correlation among leaf length,leaf width and leaf shape index.The correlation among leaf red component,blue component,green component and leaf chlorophyll reference value reached extremely significant or significant level.The chlorophyll reference value,leaf red component and green component was extremely significant negative correlation.Some phenotypes of F_(1) generation of E.ulmoides leaf were mutually restricted,and some phenotypes have relatively stable relationship,but the correlation were complicated.Twenty-nine plants with stable leaf phenotypes were obtained,among which the most stable plants were H8−8,H8−63 and H8−66.

关 键 词：杜仲 F_(1)子代 叶表型 隶属函数 

分 类 号：S727.3[农业科学—林木遗传育种]