核桃细菌性黑斑病病原分子鉴定及室内药剂筛选  

Identification of the Pathogen of Walnut Blight and Bactericide Screening in Laboratory

作　　者：李亚 张珍荫 肖波 杨斌[4] 赵宁 Li Ya;Zhang Zhenyin;Xiao Bo;Yang Bin;Zhao Ning(College of Life Sciences,Southwest Forestry University,Kunming Yunnan 650233,China;Forestry and Grassland Bureau of Yulong County,Lijiang Yunnan 674100,China;Chongqing Three Gorges Academy of Agricultural Science,Wanzhou Chongqing 404100,China;Yunnan Provincial Key Laboratory of Forest Disaster Warning and Control,Southwest Forestry University,Kunming Yunnan 650233,China)

机构地区：[1]西南林业大学生命科学学院,云南昆明650233 [2]玉龙县林业和草原局,云南丽江674100 [3]重庆三峡农业科学院,重庆万州404100 [4]西南林业大学云南省森林灾害预警与控制重点实验室,云南昆明650233

出　　处：《西南林业大学学报（自然科学）》2022年第5期104-110,共7页Journal of Southwest Forestry University：Natural Sciences

基　　金：云南省林业科技创新项目(2014CX05)资助。

摘　　要：为确定云南地区核桃细菌性黑斑病的病原菌并筛选防治核桃细菌性黑斑病的有效药剂,采用分子生物学方法鉴定病原菌,牛津杯法测试8种药剂对黑斑病病原菌的抗菌活性,测定8种药剂对黄单胞杆菌和成团泛菌,2种病原菌的室内毒力。结果表明:对2种病原菌均有抑菌活性的是3%噻霉酮、72%农用链霉素和16%苯甲·中生,其中3%噻霉酮和72%农用链霉素对成团泛菌和黄单胞杆菌的EC50分别为3.89、9.97 mg/mL和4.93、14.94 mg/mL,有着较强的抑制作用。In order to determine the pathogen of walnut blight in Yunnan area and to screen the effective agent for the control of walnut blight.Using molecular biology method to identify pathogenic bacteria and Oxford Cup method to test the antibacterial activity of 8 agents against pathogenic bacteria of walnut blight,and to determine the indoor virulence of 8 agents against Xanthomonas arboricola and Pantoea agglomerans.The results showed that 3%thiamethoxone,72%agricultural streptomycin and 16%benzoate were the most effective antimicrobial agents against both pathogens.Among them,3%thiamethoxone and 72%agricultural streptomycin had strong bacteriostatic effect on both X.arboricola and P.agglomeras.The EC50 values of this 2 antiseptic to P.agglomerans were 3.89–9.97 mg/mL,respectively,and those to X.arboricola were 4.93–14.94 mg/mL.

关 键 词：核桃细菌性黑斑病 黄单胞杆菌 成团泛菌 室内药剂筛选 

分 类 号：S664.1[农业科学—果树学]