5个相思树种根内生细菌群落多样性特征研究  

Diversity Characteristics of Endophytic Bacterial Communities in the Roots of 5 Acacia Species

作　　者：潘辉[1] 刘芳[2] 曾志浩 周丽丽 袁宗胜 Pan Hui;Liu Fang;Zeng Zhihao;Zhou Lili;Yuan Zongsheng(Institute of Oceanography,Minjiang University,Fuzhou Fujian 350108,China;College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou Fujian 350002,China)

机构地区：[1]闽江学院海洋研究院,福建福州350108 [2]福建农林大学生命科学学院,福建福州350002

出　　处：《西南林业大学学报（自然科学）》2022年第5期165-170,共6页Journal of Southwest Forestry University：Natural Sciences

基　　金：福建省林业科技项目([2019]16号)资助;闽江学院校级科研项目(MYK19027)资助。

摘　　要：采集了厚荚相思、卷荚相思、黑木相思、直干相思、马占相思共5种植物的根部样本,通过16S rRNA高通量测序,对不同种相思树中根内生细菌的群落结构进行解析。比较5种相思树种根内生细菌群落多样性,在纲水平上,厚荚相思、卷荚相思、黑木相思和直干相思的根内生优势菌为Alphaproteobacteria,而马占相思的根内生细菌中,则以Gammaproteobacteria占主导地位。在目水平上,厚荚相思、卷荚相思、直干相思和黑木相思的根内生细菌优势菌为Rhizobiales,马占相思的根内生细菌则以Rhizobiales、Enterobacteriales和unidentified_Gammaproteobacteria占主导地位。在科水平上,厚荚相思、卷荚相思、直干相思和黑木相思的根内生细菌中优势菌为Xanthobacteraceae,马占相思的根内生细菌中,则以Enterobacteriaceae占主导地位。不同种相思树根内生细菌群落在纲、目、科水平上均存在较大差异性,与其他4种相思树种的优势菌群相比,马占相思表现出一定的特异性。We collected root samples of Acacia crassicarpa,Acacia cincinnata,Acacia melanoxylon,Acacia mearnsil,and Acacia mangium.And through 16S rRNA high-throughput sequencing and statistical analysis,the diversity of endophytic bacterial communities in the roots of 5 Acacia species was compared.At the Class level,the dominant endophytic bacteria in the roots of A.crassicarpa,A.cincinnata,A.melanoxylon and A.mearnsil are Alphaproteobacteria,while Gammaproteobacteria dominates the endophytic bacteria in the roots of A.mangium.At the Order level,the dominant endophytic bacteria in the roots of A.crassicarpa,A.cincinnata,A.mearnsil and A.melanoxylon are Rhizobiales,while Rhizobiales,Enterobacteriales and Unidentified_Gammaproteobacteria dominate the root endophytic bacteria of A.mangium.At the Family level,the dominant endophytic bacteria in the roots of A.crassicarpa,A.cincinnata,A.mearnsil,and A.melanoxylon are Xanthobacteraceae,while Enterobacteriaceae dominates the root endophytic bacteria of A.mangium.The endophytic bacterial communities in the roots of different Acacia species are quite different at the level of Class,Order and Family.Compared with the dominant bacterial communities of the other 4 Acacia species,A.mangium showed specificity.

关 键 词：相思树种 内生细菌 群落结构 多样性 厚荚相思 卷荚相思 黑木相思 直干相思 马占相思 

分 类 号：S792[农业科学—林木遗传育种]