HPLC-DAD法测定益脑胶囊中3,6′-二芥子酰基蔗糖、五味子醇甲和人参皂苷Rb_(1)  

Determination of 3,6′-dimustard acyl sucrose,schisandrin A,and ginsenoside Rb1 in Yinao Capsules by HPLC-DAD

作　　者：王怀斌 刘春 李明杰 WANG Huai-bin;LIU Chun;LI Ming-jie(Department of Pharmacy,3201 Hospital,Hanzhong 723000,China;Quality Control Center,3201 Hospital,Hanzhong 723000,China)

机构地区：[1]三二〇一医院药学部,陕西汉中723000 [2]三二〇一医院质控科,陕西汉中723000

出　　处：《现代药物与临床》2022年第9期1997-2000,共4页Drugs & Clinic

摘　　要：目的建立HPLC-DAD法同时测定益脑胶囊中3,6′-二芥子酰基蔗糖、五味子醇甲和人参皂苷Rb_(1)的方法。方法采用Agilent Eclipe Zorbax XDB C_(18)柱(250 mm×4.6 mm,5μm);流动相:0.1%磷酸溶液–乙腈,梯度洗脱;检测器:DAD检测器;检测波长:203 nm(人参皂苷Rb_(1))、249 nm(五味子醇甲)、321 nm(3,6′-二芥子酰基蔗糖);体积流量:1.0 mL/min;柱温:室温;进样量:10μL。结果3,6′-二芥子酰基蔗糖、五味子醇甲和人参皂苷Rb_(1)分别在0.193~19.300、0.299~29.910、0.189~188.600μg/mL线性关系良好,平均回收率分别为99.5%、99.7%、98.3%,RSD值分别为1.2%、1.1%、0.7%。结论本法简便、准确、快速,适用于益脑胶囊中3,6′-二芥子酰基蔗糖、五味子醇甲和人参皂苷Rb_(1)的测定,为完善该产品的质量标准完善提供参考。Objective To establish an HPLC method to simultaneously determine the contents of 3,6'-dimustard acyl sucrose,schisandrin A,and ginsenoside Rb_(1) in Yinao Capsules.Methods The separation was carried out on Agilent Eclipe Zorbax XDB C_(18) column(250 mm×4.6 mm,5μm).Detector was ued as DAD detector.The mobile phase was 0.1%phosphoric acid solution-acetonitrile with gradient elution.The detection wavelength was set at 321 nm(3,6'-dimustard acyl sucrose),249 nm(schisandrin A),and 203 nm(ginsenoside Rb_(1)).The volume flow was 1.0 mL/min,column temperature was room temperature,and injection volume was 10μL.Results There had good linear relationships for 3,6'-dimustard acyl sucrose,schisandrin A,and ginsenoside Rb_(1) within the ranges of 0.193—19.300,0.299—29.910,and 0.189—188.600μg/mL,whose average recoveries were 99.5%,99.7%,and 98.3%with RSD values of 1.2%,1.1%,and 0.7%,respectively.Conclusion The method is simple,accurate,and rapid,which is suitable for the quality control for Yinao Capsules,and provides reference for improvement of quality control of the product.

关 键 词：益脑胶囊 3 6′-二芥子酰基蔗糖 五味子醇甲 人参皂苷Rb1 HPLC-DAD 

分 类 号：R286.02[医药卫生—中药学]