膝骨关节炎不同证候患者关节液Smad2浓度特点及其与证候程度间的相关性研究  

Study on the concentration characteristics of Smad2 in synovial fluid of patients with different syndromes of knee osteoarthritis and its correlation with the degree of syndrome

作　　者：叶超[1] 陈婧 刘向春[1] 亓建[1] 吴红飞 王悦 王凯丽 赵迎妍 王庆甫 YE Chao;CHEN Jing;LIU Xiang-chun;QI Jian;WU Hong-fei;WANG Yue;WANG Kai-li;ZHAO Ying-yan;WANG Qing-fu(Dongzhimen Hospital,Bejing University of Chinese Medicine,Beijing 100700,China;Beijing University of Chinese Medicine Third Affiliated Hospital,Bejing 100029,China)

机构地区：[1]北京中医药大学东直门医院,北京100700 [2]北京中医药大学第三附属医院,北京100029

出　　处：《中华中医药杂志》2022年第10期5989-5992,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金青年科学基金项目(No.82104710),国家自然科学基金面上项目(No.81874475)。

摘　　要：目的:研究膝骨关节炎肝肾亏虚证和痰瘀互结证患者关节液Smad2的浓度特点及其与证候程度间的相关性。方法:留取并测定66例膝骨关节炎不同证候患者关节液Smad2的浓度、Kellgren-Lawrence(K-L)分级标准量化影像学程度分级、中医证候评分量表量化证候程度。结果:膝骨关节炎痰瘀互结证和肝肾亏虚证患者K-L分级标准量化比较上差异无统计学意义;膝骨关节炎肝肾亏虚证患者关节液Smad2的浓度水平显著低于痰瘀互结证患者(P<0.05);膝骨关节炎肝肾亏虚证患者关节液Smad2浓度水平与中医证候评分呈负相关(r~2=0.264,P<0.05)。结论:膝骨关节炎不同证候患者关节液Smad2浓度特点不同,其中肝肾亏虚证患者关节液Smad2浓度能反映其证候的严重程度,其作用机制可能与转化生长因子β(TGF-β)/Smad信号通路受到抑制有关。Objective:To explore the characteristics of synovial fluid Smad2 concentration and its correlation with the degree of syndromes in patients with knee osteoarthritis in liver-kidney deficiency syndrome and stagnation of phlegm and blood stasis syndrome.Methods:Sixty-six patients with different syndromes of knee osteoarthritis were tested for Smad2 concentration in synovial fluid,Kellgren-Lawrence(K-L)grading standard to quantify imaging degree,and TCM syndrome scoring scale to quantify syndrome degree.Results:There was no significant difference in the quantitative comparison of K-L grading standard between patients with stagnation of phlegm and blood stasis syndrome and liver-kidney deficiency syndrome in knee osteoarthritis;The concentration of Smad2 in synovial fluid of patients with knee osteoarthritis and liver-kidney deficiency syndrome was lower than that of patients with stagnation of phlegm and blood stasis syndrome(P<0.05);The level of Smad2 in synovial fluid of knee osteoarthritis patients with liver-kidney deficiency syndrome was negatively correlated with TCM syndrome score(r~2=0.264,P<0.05).Conclusion:The concentration of Smad2 in the synovial fluid of patients with different syndromes of knee osteoarthritis is different.The synovial fluid Smad2 concentration in patients with liver-kidney deficiency syndrome can reflect the severity of the syndrome,and its mechanism may be related to the inhibition of TGF-β/Smad signaling pathway.

关 键 词：膝骨关节炎 证候 SMAD2 关节液 肝肾亏虚 痰瘀互结 相关性 

分 类 号：R274.9[医药卫生—中西医结合]