近红外H_(2)S荧光探针研究进展  

Research Progress of Near-Infrared Fluorescent Probes for Hydrogen Sulfide

作　　者：宋江涛 袁跃华[1] 朱永军[1] 王玉珍[1] 田茂忠[1] 冯锋[1] SONG Jiang-tao;YUAN Yue-hua;ZHU Yong-jun;WANG Yu-zhen;TIAN Mao-zhong;FENG Feng(College of Chemistry and Chemical Engineering,Shanxi Provincial Key Laboratory of Chemical Biosensing,Shanxi Datong University,Datong 037009,China)

机构地区：[1]山西大同大学化学与化工学院,化学生物传感山西省重点实验室,山西大同037009

出　　处：《光谱学与光谱分析》2022年第11期3321-3329,共9页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(21375083,21175085);山西省基础研究计划项目(20210302123479,202103021224312);大同市重点研发计划项目(2019019);山西大同大学研究生教育创新项目(20CX06)资助。

摘　　要：硫化氢(H_(2)S)是一种具有臭鸡蛋气味的无色气体,其不但存在于外界环境中,而且是继一氧化氮(NO)和一氧化碳(CO)之后生物系统中第三种重要的内源性气体信号分子,近年来因其对人类健康和疾病的影响而越来越受关注。H_(2)S可以作为抗氧化剂清除人体内的活性氧(ROS)和活性氮(RNS),参与多种细胞的生理反应,包括细胞凋亡、血管舒张、神经调节等,是多种组织中的细胞保护剂和气体传递剂。现代医学研究已证实硫化氢含量与糖尿病、阿尔茨海默病、帕金森病等特定疾病密切相关,但人们尚不清楚H_(2)S影响细胞信号传导和其他生理事件的具体分子机制,因此开发用于可视化内源性H_(2)S的方法、研究其在细胞和生物体中的动态分布将具有非常重要的意义。目前检测H_(2)S浓度的近红外(NIR)荧光探针是一个研究的热点,这是因为在生物样本分析时近红外荧光探针具有许多显著的优点:光损伤更小、能穿透更深的组织、背景自荧光干扰低。在分子水平上,H_(2)S表现出独特的化学特性,既是良好的还原剂,又是良好的亲核试剂,用于H_(2)S选择性检测的近红外荧光探针设计策略主要包括叠氮化物和硝基的还原、亲核进攻、加成反应等。该文章综述了近三年近红外H_(2)S荧光探针的设计合成、识别机理、探针的性能及其在细胞或生物体中的荧光成像研究与最新进展,最后,对该类探针的未来研究方向和发展趋势进行了展望。Hydrogen sulfide(H_(2)S) is a colorless gas with the unpleasant smell of rotten eggs. It not only exists in the environment but was also considered the third important endogenous gaseous transmitter following nitric oxide(NO) and carbon monoxide(CO) in biological systems. H_(2)S has recently attracted more attention for contributing to human health and disease. H_(2)S has important biological functions and has been recognized as a cytoprotectant and gasotransmitter in many tissue types, including mediating vascular tone in blood vessels and neuromodulation in the brain. Hydrogen sulfide concentration has been demonstrated to be closely correlated with particular diseases in modern medical research, such as diabetes, Alzheimer’s disease and Parkinson’s disease. The molecular mechanisms by which H_(2)S affects cell signaling and other physiological events remain unclear. Therefore, it is necessary to develop highly sensitive and selective methods for detecting the concentration of H_(2)S in living cells and organisms. The near-infrared fluorescent probe for detecting H_(2)S has been the research hotspot. Near-infrared(NIR) fluorescent probes have several significant advantages for imaging applications in vivo: negligible photodamage, deep tissue penetration, and low interference from background auto fluorescence. Many new methods for visualizing H_(2)S in living systems have been reported. At the molecular level, H_(2)S exhibits unique chemical characteristics, acting as a good reducing agent and a good nucleophile. Thus the main strategies used in NIR fluorescent probe development for H_(2)S detection include azide and nitro group reduction, nucleophilic attack, addition reaction, etc. Herein, the design and synthesis, recognition mechanism, properties of NIR fluorescent probes for H_(2)S and their fluorescence imaging in cells and organisms and the latest research progress reported in recent three years have been reviewed. Finally, in our opinion, the future research direction and development trend of this ki

关 键 词：近红外 硫化氢 荧光探针 识别机理与性能 生物成像 研究进展 

分 类 号：O657.3[理学—分析化学]