溶磷内生菌的筛选鉴定及其对薏苡生长发育的影响  

Identification and Characterization of Phosphate-solubilizing Endophytes in Coix lacryma-jobi L.

作　　者：普凤雅 谷书杰 何永宏[2] 陈崧林 杨志清[1,3,4] PU Fengya;GU Shujie;HE Yonghong;CHEN Songlin;YANG Zhiqing(College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming,Yunnan 650201,China;College of Plant Protection,Yunnan Agricultural University,Kunming,Yunnan 650201,China;Key Laboratory of Medicinal Plant Biology of Yunnan Province,Yunnan Agricultural University,Kunming,Yunnan 650201,China;National&Local Joint Engineering Research Center on Germplasm Innovation&Utilization of Chinese Medicinal Materials in Southwestern China,Yunnan Agricultural University,Kunming,Yunnan 650201,China)

机构地区：[1]云南农业大学农学与生物技术学院,云南昆明650201 [2]云南农业大学植物保护学院,云南昆明650201 [3]云南农业大学云南省药用植物生物学重点实验室,云南昆明650201 [4]云南农业大学西南中药材种质创新与利用国家地方联合工程研究中心,云南昆明650201

出　　处：《福建农业学报》2022年第7期946-953,共8页Fujian Journal of Agricultural Sciences

基　　金：云南省科技计划项目(2014RE002)。

摘　　要：【目的】筛选具有溶磷能力的薏苡内生细菌并明确其类型,为后续研制生物菌肥提供优良菌株资源。【方法】以播种25 d的薏苡幼苗为试验材料,采用研磨稀释涂板法从薏苡的根、茎、叶中分离筛选具有溶磷能力的薏苡内生菌,探索该类菌溶解有机磷、无机磷、分泌磷酸酶的能力;通过盆栽试验探究溶磷内生菌对薏苡幼苗的促生长作用。【结果】筛选到溶解有机磷细菌8株,溶解无机磷细菌5株。其中,编号为R24的菌株溶解有机磷能力最强,摇瓶5 d后,上清液可溶性磷含量为37.20mg·L^(−1);编号为L21的菌株溶解无机磷能力最强,摇瓶5 d后,上清液可溶性磷含量为62.93 mg·L^(−1).2株溶磷菌及等比例混合菌液能够促进盆栽薏苡的株高、茎粗、分蘖数,促生长能力从大到小为:L21>R24>混合菌液[V(R24)∶V(L21)=1∶1]。通过16S rDNA序列鉴定,R24为短小芽孢杆菌,L21为贝莱斯芽孢杆菌。【结论】溶磷菌L21和R24对薏苡生长有显著促进作用。【Objective】 Screening bacteria with phosphate-solubilizing ability in Coix were identified and provide excellent strain resource for bio-fertilization agent. 【Methods】 Endophytic bacteria were isolated and purified from specimens the roots, stems, and leaves of 25-d-old seedlings of Coix lacryma-jobi L. Abilities of the isolates in dissolving organophosphate and inorganic phosphate as well as in secreting phosphatase were determined. In a pot experiment, effects of two selected isolates on the growth of Coix seedlings were examined.【 Results】 Eight strains of bacteria capable of dissolving organophosphate and 5 of degrading inorganic phosphate were isolated. In the 5-d culture supernatants, the highest concentration of phosphorous converted from organophosphate at 37.20 mg·L^(−1) was reached by R24, and that from inorganic phosphate at 62.93 mg·L^(−1) by L21. The enhancing effects on plant height, stem girth, and tillering number of the potted Coix seedlings by each and mixture in equal proportion of the two supernatants ranked in the order of L21>R24>mixed bacterial liquid[ V(R24)∶ V(L21)=1∶1]. The 16S rDNA sequence phylogenetic analysis identified R24 as Bacillus pumilus and L21 as B. velezensis. 【Conclusion】 The phosphobacteria L21 and R24 displayed a significant effect in promoting the growth of Coix seedlings.

关 键 词：薏苡 内生菌 溶磷细菌 溶磷能力 鉴定 

分 类 号：S182[农业科学—农业基础科学]