铁掺杂蒙脱石纳米酶的制备与应用  

Preparation and Application of Colorimetric Sensor Based on Ferric Doping-Montmorillonite Nanozyme

作　　者：田路远 汪浩 唐爱东 杨华明[1,2,3,5,6] TIAN Luyuan;WANG Hao;TANG Aidong;YANG Huaming(Engineering Research Center of Nano-Geomaterials of Ministry of Education,China University of Geosciences,Wuhan 430074,China;Faculty of Materials Science and Chemistry,China University of Geosciences,Wuhan 430074,China;Key Laboratory of Functional Geomaterials in China Nonmetallic Minerals Industry,China University of Geosciences,Wuhan 430074,China;School of Chemistry and Chemical Engineering,Central South University,Changsha 410083,China;School of Minerals Processing and Bioengineering,Central South University,Changsha 410083,China;Hunan Key Lab of Mineral Materials and Application,Central South University,Changsha 410083,China)

机构地区：[1]中国地质大学(武汉)纳米矿物材料及应用教育部工程研究中心 [2]中国地质大学(武汉)材料与化学学院 [3]中国非金属矿行业矿物功能材料重点实验室,中国地质大学(武汉),武汉430074 [4]中南大学化学化工学院,长沙410083 [5]中南大学资源加工与生物工程学院,长沙410083 [6]中南大学矿物材料及其应用湖南省重点实验室,长沙410083

出　　处：《硅酸盐学报》2022年第10期2762-2769,共8页Journal of The Chinese Ceramic Society

基　　金：中国博士后科学基金(2020M682518);湖北省博士后创新研究计划(265053)。

摘　　要：纳米酶可以快速灵敏地检测生物标记物,有效防止疾病的爆发,但是纳米酶的催化活性低、对底物亲和力差,严重地制约了其检测性能。蒙脱石是一种具有典型的2:1型层状结构的硅酸盐黏土矿物,利用其良好的吸附能力和阳离子交换能力,将Fe^(3+)锚定在蒙脱石的表面与层间,制备具有类过氧化物酶性质的Fe掺杂蒙脱石纳米酶,可增强对H_(2)O_(2)的亲和力,通过比色法可实现对H_(2)O_(2)的检测。结果表明:在中性条件下,H_(2)O_(2)的检测线性范围和检测限分别为20~500μmol/L和5.8μmol/L。这种蒙脱石纳米酶在分析检测和疾病诊断领域具有良好的应用前景。Nanozyme can quickly and sensitively detect biomarkers to prevent the outbreak of diseases.However,the low catalytic activity and poor affinity for substrates restrict the detection performance.Montmorillonite,as a typical 2:1 layered silicate clay mineral,has a good adsorption and cation exchange ability.Thus,Fe^(3+) can be anchored into the layers to prepare Fe doping montmorillonite nanozyme with a peroxidase-like property,enhancing the affinity for H_(2)O_(2) and realizing the colorimetric detection of H_(2)O_(2).The results show that the linear range and detection limit of H_(2)O_(2) are 20-500μmol/L and 5.8μmol/L under neutral conditions,respectively.The colorimetric method for silicate clay mineral nanozyme has a good application prospect in the fields of analytical detection and disease diagnosis.

关 键 词：纳米酶 蒙脱石 类过氧化物酶 铁掺杂 比色法 分析检测 

分 类 号：TB332[一般工业技术—材料科学与工程]