circ_0010729靶向miR-654-3p介导高糖诱导的肾小球系膜细胞凋亡及炎症反应  

circ_0010729 targeting miR-654-3p mediates glomerular mesangial cell apoptosis and inflammation induced by high glucose

作　　者：陈颖 徐爽 王炟 CHEN Ying;XU Shuang;WANG Da(Health Care Room of the Second Outpatient Department,Northern Theater General Hospital,Shenyang 110015;Department of Endocrinology,Northern Theater General Hospital,Shenyang 110015,China)

机构地区：[1]北部战区总医院第二派驻门诊部保健室,沈阳110015 [2]北部战区总医院内分泌科,沈阳110015

出　　处：《临床与病理杂志》2022年第10期2335-2341,共7页Journal of Clinical and Pathological Research

摘　　要：目的:探讨circ_0010729对高糖诱导的肾小球系膜细胞损伤的影响。方法:将人肾小球系膜细胞HMCL分为对照组、高糖(high glucose,HG)组、HG+si-NC组、HG+si-circ_0010729组、H G+m i R-N C组、 HG+miR-654-3p组、HG+si-circ_0010729+anti-miR-NC组、HG+si-circ_0010729+anti-miR-654-3p组。Real-time PCR检测circ_0010729和miR-654-3p的表达水平;流式细胞术检测细胞凋亡;ELISA检测白细胞介素-6(interleukin-6,IL-6)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)水平;双荧光素酶报告实验验证circ_0010729和miR-654-3p的靶向关系。结果:与对照组比较,HG组circ_0010729表达水平升高,miR-654-3p表达水平降低,细胞凋亡率升高,TNF-α、IL-6水平升高(P<0.05)。抑制circ_0010729表达或过表达miR-654-3p后,高糖诱导的人肾小球系膜细胞中细胞凋亡率降低,TNF-α、IL-6水平降低(P<0.05)。circ_0010729靶向调控miR-654-3p;干扰miR-654-3p表达逆转了抑制circ_0010729表达对高糖诱导的人肾小球系膜细胞凋亡及炎症反应的作用。结论:抑制circ_0010729表达可能通过靶向上调miR-654-3p表达抑制高糖诱导的肾小球系膜细胞凋亡及炎症反应。Objective:To investigate the effect of circ_0010729 on high glucose-induced mesangial cell injury.Methods:Human glomerular mesangial cells HMCL were divided into a control group,a high glucose(HG)group,a HG+miR-NC group,a HG+miR-654-3p group,a HG+si-NC group,a HG+si-circ_0010729 group,a HG+si-circ_0010729+anti-miR-NC group,and a HG+si-circ_0010729+anti-miR-654-3p group.Real-time PCR was performed to detect the expression levels of miR-654-3p and circ_0010729;flow cytometry was performed to detect the apoptosis of glomerular mesangial cells;ELISA was performed to detect interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)levels;dual luciferase report experiment was used to verify the targeting relationship between circ_0010729 and miR-654-3p.Results:Compared with the control group,the circ_0010729 expression was increased,the miR-654-3p expression was decreased,the apoptosis rate was increased,the TNF-αand IL-6 levels were increased in the HG group(P<0.05).After inhibiting the expression of circ_0010729 or overexpression of miR-654-3p,the apoptosis rate of human glomerular mesangial cells induced by high glucose was decreased,the levels of TNF-αand IL-6 were decreased(P<0.05).circ_0010729 targeted and regulated miR-654-3p;interfering with the expression of miR-654-3p reversed the effect of inhibiting the expression of circ_0010729 on the apoptosis and inflammation of human glomerular mesangial cells induced by high glucose.Conclusion:Inhibiting the expression of circ_0010729 may inhibit the apoptosis and inflammation of glomerular mesangial cells induced by high glucose through targeting up-regulation of miR-654-3p expression.

关 键 词：circ_0010729 miR-654-3p 肾小球系膜细胞 凋亡 炎症反应 

分 类 号：R587.2[医药卫生—内分泌] R692.9[医药卫生—内科学]