电压扰动对EAD代谢通量中微生物与关键酶活性的影响  

Effects of voltage perturbation on the activities of microorganisms and key enzymes in EAD metabolic flux

作　　者：刘海波 王楠 刘洪周 陈铁柱 李建昌[1] LIU Haibo;WANG Nan;LIU Hongzhou;CHEN Tiezhu;LI Jianchang(School of Energy and Environmental Sciences,Yunnan Normal University,Kunming 650500,Yunnan,China)

机构地区：[1]云南师范大学能源与环境科学学院,云南昆明650500

出　　处：《化工学报》2022年第10期4603-4612,共10页CIESC Journal

基　　金：国家自然科学基金项目(21968038)。

摘　　要：为探究电压扰动对EAD代谢通量中微生物与关键酶活性的影响,实验采用单室EAD反应器,以电解电压为扰动变量,利用CNA构建代谢模型,对扰动前后微生物群落、酶活水平及通量变化进行讨论。结果表明,电压扰动后,产甲烷途径偏向于氢营养型产甲烷,约占38.5%,且0.6 V扰动时表现出最佳的甲烷通量,为0.5222 g。EAD体系中的H_(2)的主要来源是阴极和NADH,电解电压的扰动会影响H_(2)的产生,进而会影响产甲烷通量。在0.6 V扰动后,生物膜中Trichloromonas的相对丰度高达40.2%,分别是1.0 V和1.4 V的1.52倍和1.13倍,同时CoI、PTA、AK与CoF_(420)均表现出最佳的酶活水平,关键酶活水平和Trichloromonas的相对丰度与产甲烷代谢通量表现出较好的一致性,而阳极生物膜中微生物多样性也是影响EAD产甲烷通量的重要因素。In order to explore the effect of voltage perturbation on the activities of microorganisms and key enzymes in the metabolic flux of the electrochemical anaerobic digestion(EAD),a single-chamber EAD reactor was used in the experiment,the electrolysis voltage was used as the perturbation variable,and a metabolic model was constructed by using CNA.Quantitative changes are discussed.The results showed that after the voltage disturbance,the methanogenesis pathway was biased towards hydrogenotrophic methanogenesis,accounting for about 38.5%,and the 0.6 V disturbance showed the best methane flux of 0.5222 g.The main sources of H_(2) in the EAD system are the cathode and NADH,and the perturbation of the electrolysis voltage will affect the production of H_(2),which in turn will affect the methane production flux.After 0.6 V perturbation,the relative abundance of Trichloromonas in the biofilm was as high as 40.2%,which was 1.52 times and 1.13 times that of 1.0 V and 1.4 V,respectively.Meanwhile,CoI,PTA,AK and CoF_(420) all showed the best enzyme activity levels.The activity levels of key enzymes and the relative abundance of Trichloromonas showed good consistency with the methanogenesis flux,andthemicrobialdiversityintheanodebiofilm wasalsoanimportantfactoraffecting the methanogenesis flux in EAD.

关 键 词：生物能源 电化学厌氧消化 微生物群落 代谢 酶 

分 类 号：O646[理学—物理化学] Q936[理学—化学] S216[生物学—微生物学]