Comparison of 16S rRNA Gene Primers on Studying Microbial Community Composition in Bottom Water and Sediment of Artificial Reefs in Laoshan Bay, China  

作　　者：FANG Guangjie YU Haolin SHENG Huaxiang TANG Yanli LIANG Zhenlin 

机构地区：[1]Fisheries College,Ocean University of China,Qingdao 266003,China [2]Marine College,Shandong University,Weihai 264200,China

出　　处：《Journal of Ocean University of China》2022年第5期1313-1322,共10页中国海洋大学学报（英文版）

基　　金：funded by the Project of National Key R&D Program of China(No.2019YFD0901302).

摘　　要：Marine microorganisms are indispensable regulators of nutrient cycling and energy flow,which are crucial for artificial reefs(ARs)ecosystems.However,little is known about the microbial communities in the bottom water and sediment of ARs.Studies of microbial diversities have greatly advanced due to the development of high-throughput sequencing technologies,whereas the results may vary significantly due to the primers’choice.This study investigated the influences of two 16S ribosomal RNA gene primer choices(V4 and V3-V4)on microbial community compositions and structures.The results showed that the taxonomic assignment de-tected by primer V3-V4 was higher compared with that obtained by primer V4,whereas microbial community compositions had strong correlations between the two primers.Microbial beta diversities of ARs can be uncovered by both primers,but the relation-ships between communities and environmental parameters were inconsistent.The performances of the two primers for water samples were highly consistent,but the inconformity was evident for sediment samples.Given the relatively lower taxonomic classification of primer V4 for sediment samples,primer V3-V4 was recommended for later studies.With the development and advancement of ARs in China,our findings provide a meaningful reference for ecologists focusing on the microbial diversities and ecological functions of these artificial habitats in the future.

关 键 词：16S rRNA hypervariable region ARs bacteria ARCHAEA 

分 类 号：S953.1[农业科学—水产养殖]