Transcriptome Profile Based on Protein-Protein Interaction Networks Provides a Set of Core Genes for Understanding the Metabolic Mechanisms of the Egg-Protecting Behavior in Amphioctopus fangsiao  

作　　者：BAO Xiaokai LI Zan ZHANG Jianbai LI Yan CHEN Xipan WANG Weijun SUN Guohua XU Xiaohui LIU Xiumei FENG Yanwei YANG Jianmin 

机构地区：[1]School of Agriculture,Ludong University,Yantai 264025,China [2]Yantai Marine Economic Research Institute,Yantai 264004,China [3]College of Life Sciences,Yantai University,Yantai 264005,China

出　　处：《Journal of Ocean University of China》2022年第5期1323-1333,共11页中国海洋大学学报（英文版）

基　　金：supported by the earmarked fund for the Modern Agro-industry Technology Research System(No.CARS-49);the Natural Science Foundation of Shan-dong Province(No.ZR2019BC052);the National Natural Science Foundation of China(No.42006077).

摘　　要：Marine organisms cannot grow and reproduce without proper metabolic regulation.Within a metabolic network,problems with a given link will affect the normal life activities of the organism.Many metabolic mechanisms associated with behaviors of Am-phioctopus fangsiao are still unclear.Moreover,as a factor affecting the normal growth of A.fangsiao,egg protection has rarely been considered in previous behavioral studies.In this research,we analyzed the transcriptome profile of gene expression in A.fangsiao egg-unprotected larvae and egg-protected larvae,and identified 818 differentially expressed genes(DEGs).We used GO and KEGG enrichment analyses to search for metabolism-related DEGs.Protein-protein interaction networks were constructed to examine the interactions between metabolism-related genes.Twenty hub genes with multiple protein-protein interaction relationships or that were involved in multiple KEGG signaling pathways were obtained and verified by quantitative RT-PCR.We first studied the effects of egg protection on the metabolism of A.fangsiao larvae by means of protein-protein interaction networks,and the results provide va-luable gene resources for understanding the metabolism of invertebrate larvae.The data serve as a foundation for further research on the egg-protecting behavior of invertebrates.

关 键 词：Amphioctopus fangsiao egg-protecting behavior TRANSCRIPTOME protein-protein interaction networks METABOLISM 

分 类 号：Q953[生物学—动物学]