Immobilization of Chitosanase on Magnetic Nanoparticles: Preparation, Characterization and Properties  

作　　者：CHENG Yimeng LI Zhaoyue SUN Huihui ZHAO Ling LIU Zhen CAO Rong LIU Qi MAO Xiangzhao 

机构地区：[1]College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China [2]Department of Food Engineering and Nutrition,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China [3]Laboratory for Marine Drugs and Bioproducts of Qingdao,National Laboratory for Marine Science and Technology,Qingdao 266237,China

出　　处：《Journal of Ocean University of China》2022年第5期1381-1388,共8页中国海洋大学学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(No.2019 YFD0901902);the National Natural Science Foundation of China(No.31801574);the Central Public-interest Scientific Institution Basal Research Fund,YSFRI,CAFS(No.20603022021020);Qingdao Science and Technology Demonstration and Guidance Project for Benefiting the People(No.20-3-4-28-nsh).

摘　　要：Chitosanase could cleaveβ-1,4-linkage of chitosan to produce chitooligosaccharides(COS)with diverse biological activities.However,there are many limitations on the use of free chitosanase in industrial production.Enzyme immobilization is generally considered a valuable strategy in industrial-scale applications.In this study,the chitosanase Csn-BAC from Bacillus sp.MD-5 was immobilized on Fe_(3)O_(4)-SiO_(2) magnetic nanoparticles(MNPs)to enhance its properties,which could be recovered easily from reaction media using magnetic separation.The activities of Csn-BAC immobilized with MNPs(MNPs@Csn-BAC)were de-termined with temperature and pH,and the thermal-and pH-stabilities,respectively.The reusability of the MNPs@Csn-BAC was determined in repeated reaction cycles.Immobilization enhanced the thermal and pH stability of Csn-BAC compared with the free enzyme.After eight reaction cycles using MNPs@Csn-BAC,the residual enzyme activity was 72.15%.Finally,the amount of COS released by MNPs@Csn-BAC was 1.86 times higher than that of the free Csn-BAC in the catalytic performance experiment.The immobilized Csn-BAC exhibits broad application prospects in the production of COS.

关 键 词：CHITOSANASE IMMOBILIZATION magnetic nanoparticles CHITOOLIGOSACCHARIDES 

分 类 号：TQ426.97[化学工程]