慢性鼻窦炎伴双侧鼻息肉的发病与RANTES基因多态性的关系分析  

Relationships between RANTES gene polymorphisms and chronic sinusitis with bilateral nasal polyps

作　　者：刘厚军 张倩[2] 程友 薛飞 许莉 吴明海 LIU Houjun;ZHANG Qian;CHENG You;XUE Fei;XU Li;WU Minghai(Department of Otorhinolaryngology&Head and Neck Surgery,Eastern Theater Command General Hospital,Nanjing 210000,Jiangsu,China;Department of Otorhinolaryngology&Head and Neck Surgery,The First Affiliated Hospital of Henan,Kaifeng 250061,Henan,China)

机构地区：[1]东部战区总医院耳鼻咽喉头颈外科,江苏南京210000 [2]河南大学第一附属医院耳鼻咽喉头颈外科,河南开封475000

出　　处：《山东大学耳鼻喉眼学报》2022年第3期165-170,共6页Journal of Otolaryngology and Ophthalmology of Shandong University

基　　金：2021年“科教强卫”项目-南京市医学重点专科(第十一周期)(SZDZK202002)。

摘　　要：目的研究苏皖地区汉族人群中,RANTES基因(rs2107538和rs2280788)位点的单核苷酸多态性(SNP)与慢性鼻窦炎伴双侧鼻息肉(CRSwBNP)之间的相关性。方法分别收集CRSwBNP患者(病例组)、健康体检者(对照组)141例、108例,样本总量249例。收集所有样本的临床资料,并采集样本患者3 mL外周血,提取样本基因,采取病例-对照的实验方法,对RANTES基因位点(rs2107538和rs2280788)进行分型检测,最后使用Logistic回归分析法对分型结果进行统计分析。结果对病例组、对照组临床资料、实验数据进行统计学分析,发现性别与年龄分布无统计学差异(P>0.05)。研究发现rs2107538位点有纯合野生型CC、杂合型CT、纯合突变型TT三类基因型;而rs2280788位点有纯合突变型GG、纯合野生型CC、杂合型CG三类基因型。其中rs2107538位点等位基因T与野生型基因C比较(P=0.037),其增加了CRSwBNP患病风险。rs2280788位点的等位基因中未发现增加CRSwBNP的患病风险。对RANTES基因的两个位点进行连锁不平衡分析,结果发现,在单倍型RANTES rs2107538/rs2280788基因型中,基因型CG在CRSwBNP中起保护作用。结论RANTES基因的rs2280788位点多态性与CRSwBNP不存在相关性,而rs2107538位点多态性与CRSwBNP患者发病易感性相关。Objective The aim of this study was to examine the relationship between single nucleotide polymorphisms of the RANTES gene(rs2107538 and rs2280788)and chronic sinusitis with bilateral nasal polyps(CRSwBNP)in the Han population of Jiangsu and Anhui regions.Methods One hundred and forty-one CRSwBNP patients(case group)and 108 healthy persons(control group)were enrolled,for a total of 249 participants.Clinical data were collected from all participants.Peripheral blood samples were collected and DNA was extracted.The RANTES gene locus(rs2107538 and rs2280788)was genotyped and the results were compared between case and control groups using logistic regression analysis.Results No significant differences in gender or age distribution were found between the case and control groups(P>0.05).CC,CT,and TT genotypes were detected at the rs2107538 locus and GG,CC,and CG genotypes were detected at the rs2280788 locus.The rs2107538 T allele was associated with a significantly increased risk of CRSwBNP(P=0.037,odds ratio[OR]=0.68,95%confidence interval[CI]=0.47-0.98).The genotype at the rs2280788 locus was not associated with an increased risk of CRSwBNP.Linkage disequilibrium analysis showed that the RANTES rs2107538/rs2280788 C/G haplotype was protective against CRSwBNP(P=0.037,OR=0.68,95%CI=0.47-0.98).Conclusion Thers2280788 polymorphism of the RANTES gene was not associated with CRSwBNP,but the rs2107538 polymorphism of the RANTES gene was associated with susceptibility to CRSwBNP.

关 键 词：RANTES基因 单核苷酸多态性 慢性鼻窦炎 鼻息肉 细胞因子 

分 类 号：R765.25[医药卫生—耳鼻咽喉科]