冬小麦lncRNA1808调控PPP限速酶(Ta6PGDH)抗寒应答研究  

Study on lncRNA1808 Regulating Cold Resistance Response of PPP Rate-Limiting Enzyme(Ta6PGDH) in Winter Wheat

作　　者：李沅珊 彭瞰看 田宇[1] 任治鹏 苍晶[1] 徐庆华[1] 王军虹[1] LI Yuanshan;PENG Kankan;TIAN Yu;REN Zhipeng;CANG Jing;XU Qinghua;WANG Junhong(College of Life Sciences,Northeast Agricultural University,Harbin,Heilongjiang 150030,China)

机构地区：[1]东北农业大学生命科学学院,黑龙江哈尔滨150030

出　　处：《麦类作物学报》2022年第9期1047-1058,共12页Journal of Triticeae Crops

基　　金：国家自然科学基金项目(31971831,3160101042,31601236)。

摘　　要：为探索lncRNA1808调控Ta6PGDH应答寒胁迫的机制,以冬小麦品种东农冬麦1号(Dn1)为材料,应用生物信息学分析了lncRNA1808和Ta6PGDH的生物学特性和功能,预测了lncRNA1808-Ta6PGDH互作关系和共表达趋势;应用real-time PCR鉴定小麦分蘖节和叶片的lncRNA1808和Ta6PGDH寒胁迫下表达谱变化。结果显示,lncRNA1808为lncRNA,不具备编码能力,富含稳定的茎环结构;lncRNA1808与Ta6PGDH的启动子序列含多种响应环境因子的顺式作用元件;预测lncRNA1808-Ta6PGDH互作并协同共表达;Dn1分蘖节和叶片的lncRNA1808和Ta6PGDH的表达谱与协同共表达分析一致,表达趋势上调。本研究初步揭示了冬小麦lncRNA1808调控PPP限速酶Ta6PGDH的抗寒应答机制。In order to explore the mechanism of lncRNA1808 regulating the response of Ta6PGDH to cold stress, the biological characteristics and functions of lncRNA1808 and Ta6PGDH were analyzed by bioinformatics, and the interaction relationship and co-expression trend of lncRNA1808-Ta6PGDH were predicted, with winter wheat variety Dongnongdongmai 1(Dn1) as material.Real-time PCR was used to identify the expression profile changes of lncRNA1808 and Ta6PGDH in tiller nodes and leaves under cold stress.The results showed that lncRNA1808 is lncRNA, which has no coding ability and is rich in stable stem-loop structure;the promoter sequences of lncRNA1808 and Ta6PGDH contain cis-acting elements that respond to environmental factors.The interaction and co-expression of lncRNA1808-Ta6PGDH were predicted. Real-time PCR identified the expression profiles of lncRNA1808 and Ta6 PGDH in tillering nodes and leaves of Dn1, which were consistent with the co-expression analysis, and the expression trend was up-regulated.This study preliminarily revealed the mechanism of lncRNA1808 regulating the cold resistance response of PPP rate-limiting enzyme Ta6PGDH.

关 键 词：冬小麦 抗寒应答 戊糖磷酸途径 6-磷酸葡萄糖酸脱氢酶 lncRNA 

分 类 号：S512.1[农业科学—作物学] S330