野生疣鼻天鹅洛菲不动杆菌的分离鉴定与药物敏感性分析  

Identification and Drug Susceptibility Analysis of Acinetobacter lwoffii from Wild Mute Swan

作　　者：彭志锋 张菡[1] 高如意 朱亚博 董青[1] 王军[1] 霍军[1] 边传周[1] PENG Zhifeng;ZHANG Han;GAO Ruyi;ZHU Yabo;DONG Qing;WANG Jun;HUO Jun;BIAN Chuanzhou(College of Veterinary Medicine,Henan University of Animal Husbandry and Economy,Zhengzhou,450046,China;Sanmenxia National Wetland Park of Swan Lake,Sanmenxia,472100,China)

机构地区：[1]河南牧业经济学院动物医药学院,郑州450046 [2]三门峡天鹅湖国家城市湿地公园,三门峡472100

出　　处：《野生动物学报》2022年第4期1089-1093,共5页CHINESE JOURNAL OF WILDLIFE

基　　金：2021年度河南省重点研发与推广专项(科技攻关)项目(212102110372);河南牧业经济学院预防兽医重点学科项目(MXK2016102);河南牧业经济学院科研创新基金资助项目(XKYCXJJ2020011);三门峡市院地院企合作项目(2020030302);河南牧业经济学院博士科研启动资金项目(2018HNUAHEDF028)。

摘　　要：为确认导致野生疣鼻天鹅(Cygnus olor)死亡的致病菌,2020年8月,从三门峡天鹅湖国家城市湿地公园病死的野生疣鼻天鹅脏器中分离到可疑致病菌株HeNSMX-1,经染色镜检、细菌MALDI-TOF鉴定和16S rDNA序列分析,确定该分离菌株为洛菲不动杆菌(Acinetobacter lwoffii)。采用纸片法分析HeNSMX-1菌株的耐药性,结果显示:对阿米卡星、加替沙星、多西环素、卡那霉素、环丙沙星、头孢噻肟、庆大霉素和氧氟沙星敏感,对阿莫西林、氨苄西林和头孢克肟耐药。To identify causative bacteria of the death of wild mute swan(Cygnus olor),the suspected causative pathogen HeNSMX-1 was isolated from liver of dead wild mute swan in the Sanmenxia National Wetland Park of Swan Lake in August 2020.Using staining microscopy,MALDI-TOF identification,16S rDNA genetic evolutionary analysis,the strain was identified as Acinetobacter lwoffii.Then,Kirby-Bauer was used to evaluate antimicrobial susceptibility of the A.lwoffii strain HeNSMX-1.The results showed the strain was sensitive to amikacin,gatifloxacin,doxycycline,kanamycin,ciprofloxacin,cefotaxime,gentamicin and ofloxacin,and resistant to amoxycillin,ampicillin,cefixime.

关 键 词：疣鼻天鹅 洛菲不动杆菌 鉴定 药敏试验 

分 类 号：S858.9[农业科学—临床兽医学]