血清miR-423-5p、miR-499-5p和miR-210-3p在慢性心力衰竭大鼠中的表达及意义  

Expression and significance of serum miR-423-5p,miR-499-5p and miR-210-3p in chronic heart failure rats

作　　者：李秀芩[1] 陈少鹏[1] 邵伟华[1] 王素星[1] 李芳[1] 姚丽霞[1] LI Xiuqin;CHEN Shaopeng;SHAO Weihua;WANG Suxing;LI Fang;YAO Lixia(Department of Geriatric Cardiovascular Diseases,Hebei General Hospital,Shijiazhuang 050051,China)

机构地区：[1]河北省人民医院老年心血管内科,石家庄050051

出　　处：《山西医科大学学报》2022年第10期1248-1252,共5页Journal of Shanxi Medical University

基　　金：河北省医学科学研究重点课题计划项目(20180031)。

摘　　要：目的探讨血清微小RNA-423-5p(miR-423-5p)、微小RNA-499-5p(miR-499-5p)、微小RNA-210-3p(miR-210-3p)在慢性心力衰竭(CHF)大鼠中的表达及意义。方法SD大鼠随机分为对照(NC)组(n=15)和CHF组(n=15),采用腹腔注射阿霉素建立CHF模型。建模6周后检测两组心功能指标[左心室舒张末期容积(LVEDV)、左心室收缩末期容积(LVESV)、左室射血分数(LVEF)]、血清miR-423-5p、miR-499-5p、miR-210-3p表达水平及氨基末端脑利钠肽前体(NTpro-BNP)、心肌肌钙蛋白I(cTnI)水平。采用Pearson法分析血清miR-423-5p、miR-499-5p、miR-210-3p与心功能指标、NTpro-BNP、cTnI相关性。结果与NC组比较,CHF组心功能指标LVEDV和LVESV升高(P<0.05),miR-423-5p、miR-499-5p和miR-210-3p表达水平升高(P<0.05),NTpro-BNP和cTnI水平升高(P<0.05),LVEF降低(P<0.05)。CHF大鼠血清miR-423-5p、miR-499-5p、miR-210-3p与LVEDV、LVESV、LVEF无明显相关性(P>0.05),而CHF大鼠血清miR-423-5p与NTpro-BNP、cTnI呈正相关(r=0.668,P=0.007;r=0.622,P=0.004),CHF大鼠血清miR-499-5p与NTpro-BNP、cTnI呈正相关(r=0.532,P=0.013;r=0.663,P=0.041),CHF大鼠血清miR-210-3p与NTpro-BNP、cTnI呈正相关(r=0.598,P=0.019;r=0.533,P=0.041)。结论CHF大鼠血清miR-423-5p、miR-499-5p、miR-210-3p表达水平升高,且与NTpro-BNP和cTnI密切相关。Objective To investigate the expression and significance of serum microRNA-423-5p(miR-423-5p),microRNA-499-5p(miR-499-5p)and microRNA-210-3p(miR-210-3p)in chronic heart failure(CHF)rats.Methods SD rats were randomly divided into control(NC)group(n=15)and CHF group(n=15).The CHF model was established by intraperitoneal injection of doxorubicin.After 6 weeks of modeling,cardiac function indexes[left ventricular end-diastolic volume(LVEDV),left ventricular end-systolic volume(LVESV),left ventricular ejection fraction(LVEF)],serum miR-423-5p,miR-499-5p,miR-210-3p expression levels,and the levels of amino-terminal precursor brain natriuretic peptide(NTpro-BNP)and cardiac troponin I(cTnI)were detected in the two groups.Pearson’s method was used to analyze the correlation between serum miR-423-5p,miR-499-5p,miR-210-3p and cardiac function indexes,NTpro-BNP and cTnI.Results Compared with NC group,LVEDV and LVESV were increased in CHF group(P<0.05),the expression levels of miR-423-5p,miR-499-5p and miR-210-3p were increased(P<0.05),and the levels of NTpro-BNP and cTnI were increased,while LVEF was decreased(P<0.05).Serum miR-423-5p,miR-499-5p and miR-210-3p levels were not significantly correlated with LVEDV,LVESV and LVEF in CHF rats(P>0.05),while serum miR-423-5p was positively correlated with NTpro-BNP and cTnI in CHF rats(r=0.668,P=0.007;r=0.622,P=0.004),serum miR-499-5p was positively correlated with NTpro-BNP and cTnI in CHF rats(r=0.532,P=0.013;r=0.663,P=0.041),and serum miR-210-3p was positively correlated with NTpro-BNP and cTnI in CHF rats(r=0.598,P=0.019;r=0.533,P=0.041).Conclusion The expression levels of miR-423-5p,miR-499-5p and miR-210-3p in serum of CHF rats are increased,and they are closely related to NTpro-BNP and cTnI.

关 键 词：慢性心力衰竭 miR-423-5p miR-499-5p miR-210-3p 

分 类 号：R541.6[医药卫生—心血管疾病]