脂肪源性干细胞在小鼠膝关节骨关节炎中的作用研究  

Effect of Adipose-Derived Stem Cells on Knee Osteoarthritis in Mice

作　　者：白冰[1] 孙铭霞[1] 孙彦豹[1] 崔万勇[1] 张小雪[1] 王友[1] BAI Bing;SU Mingxia;SUN Yanbao(Chengde Central Hospital,Hebei Chengde 067000,China)

机构地区：[1]河北省承德市中心医院骨科,河北承德067000

出　　处：《河北医学》2022年第10期1659-1663,共5页Hebei Medicine

基　　金：河北省承德市科学技术与发展计划,(编号:202102A019)。

摘　　要：目的:探讨髌上源性ASC自体移植对小鼠KOA模型中膝关节炎症和软骨退行性等级的影响及可能机制。方法:分离髌上或髌下区域的ASC,分别测定ASC的增殖及脂肪形成、成骨、软骨形成定向分化能力,复制OA小鼠模型并进行细胞移植,检测小鼠膝盖组织的GAG分析及损伤评分。结果:体外实验显示,与髌下衍生和髌下分化细胞组相比,髌上衍生的ASC组的脂肪形成,成骨及软骨形成明显增加。体内实验表明,OA诱导后所有三组的膝关节尺寸均显着增加,与对照组相比,hASC、SVF和PRP干预组均显示出膝关节增加的体积显著减少,hASC组GAG含量明显增加,OA损伤评分明显降低。结论:人类髌上脂肪垫来源的ASC通过促进KOA小鼠中膝关节的有效内源性软骨形成,促进软骨再生。Objective:To investigate the effects and possible mechanisms of suprapatellar-derived ASC autograft on knee inflammation and degenerative grade of cartilage in a mouse KOA model.Methods:ASC in the suprapatellar or infrapatellar region were isolated,ASC proliferation and fat-forming,osteogenic and cartilage-forming directed differentiation ability were measured respectively,the OA mouse model was replicated and cell transplantation was performed,and the GAG analysis and injury score of mouse knee tissue were examined.Results:In vitro experiments showed a significant increase in fat formation,osteogenesis and cartilage formation in the suprapatellar-derived ASC group compared to the infrapatellar-derived and infrapatellar-differentiated cell groups.In vivo experiments showed a significant increase in knee dimensions in all three groups after OA induction.hASC,SVF and PRP intervention groups all showed a significant decrease in increased knee volume compared to the control group,a significant increase in GAG content and a significant decrease in OA injury score in the hASC group.Conclusion:Human suprapatellar fat pad-derived ASC promotes cartilage regeneration by promoting effective endogenous cartilage formation in the knee joint in KOA mice.

关 键 词：脂肪源性干细胞 膝关节 骨关节炎 

分 类 号：R684.3[医药卫生—骨科学]