框镜鲤邻单胞菌的分离鉴定与抗菌药物筛选  

Isolation, Identification and Antibacterials Screening of Plesiomonas shigelloides from Cyprinus carpio

作　　者：王玲玲 杜苏兰[1] 侯天牧 雷连成 易提林 袁汉文[1,2,4] 张付贤[1,2,4] WANG Ling-ling;DU Su-lan;HOU Tian-mu;LEI Lian-cheng;YI Ti-lin;YUAN Han-wen;ZHANG Fu-xian(College of Animal Science,Yangtze University,Jingzhou,Hubei,434023,China;Engineering Research Center of Ecology and Agricultural Use of Wetland,Ministry of Education,Jingzhou,Hubei,434023,China;College of Veterinary Medicine,Jilin University,Changchun,Jilin,130062,China;Key Laboratory of Waterlogging Disasters and Wetland Agriculture of Hubei Province,Jingzhou,Hubei,434023,China)

机构地区：[1]长江大学动物科学学院,湖北荆州434023 [2]湿地生态与农业利用教育部工程研究中心,湖北荆州434023 [3]吉林大学动物医学学院,吉林长春130062 [4]涝渍灾害与湿地农业湖北省重点实验室,湖北荆州434023

出　　处：《动物医学进展》2022年第11期39-45,共7页Progress In Veterinary Medicine

基　　金：湖北省农业农村厅第三批现代农业产业技术体系项目(33120090);教育部湿地生态与农业利用工程研究中心项目(KFT202003)。

摘　　要：为了确定引起湖北荆州某养殖场框镜鲤患病的病原,筛选出用于控制病原感染的抗菌药物和中药。从患病框镜鲤中分离到1株优势菌QPB1,通过生理生化和分子特征进行鉴定,结合药敏试验和耐药基因检测分析菌株的抗菌药物敏感性,并从39种中药中筛选有抑菌效果的药物。结果显示,分离菌株QPB1为革兰氏阴性杆菌,其16S rRNA基因序列与类志贺邻单胞菌(MK397781.1)的序列同源性达到99.79%;药敏试验表明QPB1对庆大霉素、环丙沙星等13种抗菌药物敏感,对红霉素、哌拉西林等7种抗菌药物耐药,并携带耐药基因tetA、tetM、sulⅠ、sulⅡ;中药抑菌试验表明,丁香、连翘、黄连等9种中药对QPB1表现出明显的抑制作用。试验表明,从患病框镜鲤中分离的优势菌株QPB1为类志贺邻单胞菌,其生物学特性、耐药基因型及抗菌药物的筛选为类志贺邻单胞菌的临床诊断和精准施治提供理论依据和实践参考。In order to identify the pathogen causing the disease of Cyprinus carpio from an aquaculture farm in Jingzhou, Hubei province, and screen out antimicrobials and traditional Chinese medicines for controlling pathogenic infection, a dominant strain, QPB1,was isolated from sick Cyprinus carpio.It was identified by physiological, biochemical and molecular characteristics.The antimicrobial sensitivity of the isolate was analyzed by drug sensitivity test and drug resistance gene detection, and the bacteriostatic effect to the isolate was screened from 39 traditional Chinese medicines.The results showed that the isolate QPB1 was a Gram-negative bacillus, and 16 S rRNA gene sequence of QPB1 has a sequence homology of 99.79% with Plesiomonas shigelloides(MK397781.1).The drug resistance test showed that QPB1 was sensitive to 13 antimicrobials such as gentamicin and ciprofloxacin, and was resistant to 7 antimicrobials such as erythromycin and piperacillin, and carried drug resistance genes tetA,tetM,sulⅠ,sulⅡ.The antibacterial experiments of traditional Chinese medicines showed that nine kinds of traditional Chinese medicines such as Forsythia suspensa,Eugenia caryophyllata Thunb.and Coptis chinensis Franch.showed obvious inhibitory effects on QPB1.The results showed that the dominant strain QPB1 isolated from the diseased Cyprinus carpio is Plesiomonas shigelloides,the biological characteristics, antimicrobial resistance genotype analysis and antimicrobial drug screening of isolated strains provide theoretical basis and practical reference for clinical diagnosis and precise treatment of Plesiomonas shigelloides.

关 键 词：框镜鲤 类志贺邻单胞菌 耐药基因 药物敏感性 中药 

分 类 号：S859.3[农业科学—临床兽医学] S941.42[农业科学—兽医学]