一种新型近红外荧光探针用于Aβ40聚集体的检测  

A Novel Near-infrared Fluorescent Probe for Detection of Aβ40 Aggregates

作　　者：徐蕴泽 尧雨斯 吕光磊 李春霞[1] XU Yun-ze;YAO Yu-si;LYU Guang-lei;LI Chun-xia(Institute of Molecular Sciences Engineering,Institute of Frontier and Interdisciplinary Science,Shandong University,Qingdao 266237,China;College of Chemistry and Life Science,Zhejiang Normal University,Jinhua 321004,China)

机构地区：[1]山东大学前沿交叉科学青岛研究院,分子科学与工程研究院,山东青岛266237 [2]浙江师范大学化学与生命科学学院,浙江金华321004

出　　处：《发光学报》2022年第10期1620-1627,共8页Chinese Journal of Luminescence

基　　金：国家自然科学基金面上项目(51872263);浙江省自然科学基金重点项目(LZ19E020001);青年项目(LQ19B050003);泰山学者计划(ts20190911)资助项目。

摘　　要：淀粉样蛋白(Aβ)形成的老年斑块是阿尔茨海默症(Alzheimer’s disease,AD)最重要的病理性标志物之一,在AD的发病中起到了非常重要的作用。在AD的发病前期,Aβ40聚集体是最为主要且大量存在的蛋白形式。因此,对Aβ40聚集体的检测有利于实现对AD的早期诊断。本工作设计合成了一种具有D-π-A结构的小分子近红外荧光探针C-1用于Aβ40聚集体的荧光检测。探针C-1具有良好的荧光发射波长(λ_(cm)=640 nm),对Aβ40聚集体表现出了较好的结合能力(K_(d)=3.072μmol/L),能够实现在溶液中对Aβ40聚集体的特异性检测。除此之外,该探针合成简单,成本低廉,灵敏度较高,响应速度极快。β-Amyloid(Aβ)senile plaques are one of the most important pathological markers of Al-zheimer’s disease(AD),which plays a quite important role in the pathogenesis of AD. In the earlystage of AD,Aβ40 aggregates are the most predominant and abundant. Therefore,the detection of Aβ40 aggregates is conducive to the early diagnosis of AD. In this work,a small molecule fluores-cent probe C-1 with a D-π-A structure was designed and synthesized for the fluorescence detectionof Aβ40 aggregates. The probe C-1 displays superior fluorescence properties(λ_(cm)=640 nm) andshows remarkable binding capacity for Aβ40 aggregates(K_(d)=3. 072 μmol/L),enabling the specificdetection of Aβ40 aggregates in solution. In addition,the probe has series of advantages,includingsimple synthesis,low cost,high sensitivity and fast response.

关 键 词：荧光探针 AΒ 阿尔茨海默症 近红外 

分 类 号：TP394.1[自动化与计算机技术—计算机应用技术] TH691.9[自动化与计算机技术—计算机科学与技术]