二甲双胍改善瓦斯爆炸肾损伤的试验研究  

Experimental study on treatment effect of MET on renal injuries in gas explosion

作　　者：田林强[1] 来枫 张玥 翁孝刚[1] 姚三巧[2] 任文杰 TIAN Linqiang;LAI Feng;ZHANG Yue;WENG Xiaogang;YAO Sanqiao;REN Wenjie(Institute of Trauma and Orthopedics,Third Affiliated Hospital of Xinxiang Medical University,Xinxiang Henan 453003,China;School of Public Health,Xinxiang Medical University,Xinxiang Henan 453003,China)

机构地区：[1]新乡医学院第三附属医院创伤与骨科研究所,河南新乡453003 [2]新乡医学院公共卫生学院,河南新乡453003

出　　处：《中国安全科学学报》2022年第10期207-213,共7页China Safety Science Journal

基　　金：中国职业安全健康协会创新创业项目(CXCY-2021-11);新乡医学院第三附属医院开放课题项目(KFKTZD202104)。

摘　　要：为明确瓦斯爆炸肾损伤的特点以及二甲双胍(MET)的治疗效果,并探讨其可能的作用机制,将大鼠随机分为正常对照组(NC组)、瓦斯爆炸肾损伤组(SW组)和瓦斯爆炸肾损伤+MET治疗组(MET组),利用真实巷道建立瓦斯爆炸大鼠肾损伤模型,检测肾功能,观察肾脏组织学变化及凋亡相关分子的表达变化,并检测腺苷酸活化蛋白激酶(AMPK)和PGC-1α的表达情况。结果表明:瓦斯爆炸肾损伤组织学表现为肾小球和肾小管明显充血和水肿,伴肾功能异常;MET能够改善肾功能,减轻肾损伤,可能是通过激活AMPK及其下游分子PGC-1α,抑制细胞凋亡实现的。In order to explore characters of kidney injuries caused by gas explosion and treatment effect of MET as well as its possible action mechanism,rat samples were divided into normal control group(NC group),kidney injury caused by gas explosion group(SW group)and kidney injury caused by gas explosion treated with MET group randomly.Then,kidney injury model of rats was built by utilizing actual roadway,renal functions were tested,histological changes of kidney and express changes of apoptosis associated molecular were observed,and those of AMPK and PGC-1αwere detected.The results show that kidney injuries are normally expressed as significant congestion and edema in renal corpuscle and kidney tubules along with kidney disfunction.MET can improve renal function and decrease damages probably through activating AMPK and PGC-1α,thus inhibiting apoptosis of renal tubular epithelial cells.

关 键 词：二甲双胍(MET) 瓦斯爆炸 急性肾损伤 腺苷酸活化蛋白激酶(AMPK) 过氧化物酶增殖物激活受体γ共激活因子1α(PGC-1α) 

分 类 号：X928.9[环境科学与工程—安全科学]