黄芪多糖对Lewis荷瘤小鼠PD-1/PD-L1表达的影响  

Regulatory effects of astragalus polysaccharides on PD-1/PD-L1 expression in Lewis tumor-bearing mouse model

作　　者：李津津 杨金颖 孙芳芳 王丽[2] Li Jinjin;Yang Jinying;Sun Fangfang;Wang Li(Tianjin Wuqing District Traditional Chinese Medicine Hospital,Tianjin301700;Tianjin Second People's Hospital,Tianjin300192)

机构地区：[1]天津市武清区中医医院,天津301700 [2]天津市第二人民医院,天津300192

出　　处：《天津药学》2022年第5期9-13,37,共6页Tianjin Pharmacy

基　　金：天津市医学重点学科(专科)建设项目(No.TJYXZDXK-059B)。

摘　　要：目的:探究黄芪多糖对Lewis肺癌小鼠的抗肿瘤作用及免疫功能的影响。方法:选取40只雄性C57BL/6小鼠随机分为模型组、阳性药组、黄芪多糖低剂量组和黄芪多糖高剂量组,每组10只。各组使用Lewis细胞建立肺癌小鼠模型。造模成功后阳性对照组小鼠隔日腹腔注射顺铂2 mg/kg;黄芪多糖低剂量组每日灌胃黄芪多糖200 mg/kg;黄芪多糖高剂量组每日灌胃黄芪多糖400 mg/kg。造模给药结束后,计算各组小鼠抑瘤率、脾指数和胸腺指数;流式细胞术检测外周血T淋巴细胞亚群比率;ELISA检测血清中干扰素γ(IFN-γ)和白介素-2(IL-2)含量;Western blot法检测脾组织中程序性细胞死亡蛋白1(PD-1)、肿瘤组织中程序性细胞死亡蛋白配体1(PD-L1)蛋白表达量。结果:黄芪多糖能显著提高抑瘤率,增加肺癌小鼠脾指数和胸腺指数;流式细胞术结果表明黄芪多糖增加CD4^(+)T细胞比率及CD4^(+)/CD8^(+)T细胞比率;酶联接免疫吸附剂测定(ELISA)结果表明黄芪多糖可以升高肺癌小鼠血清中IFN-γ和IL-2含量;Western blot结果表明黄芪多糖可以降低肺癌小鼠脾组织中PD-1和肿瘤组织中PD-L1的蛋白表达量。结论:黄芪多糖可以抑制肺癌小鼠肿瘤生长,提高免疫功能是其可能机制。Objective:The aim of this study was to evaluate the antitumor mechanisms ofastragalus polysaccharides(APS)based on modulating the PD-1/PD-L1 signaling pathway.Methods:40 male C57BL/6 mice were randomly divided into model group,positive control group,APS low-dose group,and APS high-dose group,with ten mice in each group.Lewis lung cancer cells were used to establishthe tumor-bearing mouse model.After successful modeling,the positive control group mice were intraperitoneally injected with cisplatin(2 mg/kg)every other day.The APS low-dose and high-dose groups were given 200 mg/kg and 400 mg/kg APS per day by gavage,respectively.The tumor inhibition rate,spleen and thymus indexes were investigated after APS treatment to evaluate the antitumor effects of APS.In addition,the levels of IL-2 and IFN-γ in serum were measured using ELISA,the proportions of CD4^(+)and CD8^(+)T cells were measured using flow cytometry,and the expression of PD-L1 in tumor and the expression of PD-1 in the spleen were measured using western-blot.Results:APS could significantly inhibit tumor growth and increase the spleen and thymus indexes in Lewis tumor-bearing mice.The flow cytometry results showed that APS could increase the ratio of CD4^(+)/CD8^(+)T cells in the blood.The ELISA results showed that APS could also enhance the serum levels of IL-2 and IFN-γ in tumor-bearing mice.Western-blot results showed that APS treatment decreased the expression of PD-L1 in tumors and PD-1 in the spleen.Conclusion:APS can inhibit the growth of lung cancer in mice,and its possible mechanism is to improve immune function.

关 键 词：肺癌 黄芪多糖 免疫 PD1/PDL1 小鼠 

分 类 号：R965[医药卫生—药理学]