高密度脂蛋白的蛋白质组学在冠心病中的研究进展  

High-Density Lipoprotein Proteome in Coronary Artery Disease

作　　者：曾谷雨 袁晋青[1] ZENG Guyu;YUAN Jinqing(Fuwai Hospital,National Center for Cardiovascular Diseases,Chinese Academy of Medical Sciences,Chinese Academy of Medical Science&Peking Union Medical College,Beijing 100037,China)

机构地区：[1]中国医学科学院,北京协和医学院,国家心血管病中心,阜外医院,北京市100037

出　　处：《中国分子心脏病学杂志》2022年第4期4853-4857,共5页Molecular Cardiology of China

基　　金：国家自然科学基金(81770365)。

摘　　要：由于高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-C)和心血管病事件之间的关联尚存在争议,高密度脂蛋白(high density lipoprotein,HDL)的蛋白质组学分析逐渐受到关注。HDL可经超速离心、免疫亲和层析、尺寸排阻色谱、金属螯合色谱等技术从血浆中分离出来,随后可利用鸟枪法、多反应监测、平行反应监测、非数据依赖性采集等质谱技术分析HDL蛋白质组分。对HDL蛋白质组学的研究有助于发掘与冠心病发生、发展及预后相关的生物标志物,探究HDL的功能,评估心血管事件发生风险,并指导冠心病的诊断和治疗。HDL proteome is gaining increased attention due to the controversial association between HDL cholesterol and cardiovascular events.Isolation methodology of HDL includes ultracentrifugation,immunoaffinity chromatography,size exclusion chromatography,metal chelate chromatography,Shotgun method,multiple reaction monitoring,parallel reaction monitoring,data independent acquisition and other techniques are available for HDL protein analysis.The HDL proteome can help discover new biomarkers of coronary artery disease,explore HDL function,evaluate the risk of adverse cardiovascular events and guide the diagnosis and treatment of coronary artery disease.

关 键 词：高密度脂蛋白 蛋白质组学 质谱技术 冠心病 

分 类 号：R541.4[医药卫生—心血管疾病]