CRISPR-mediated mutagenesis of the odorant receptor co-receptor(Orco)gene disrupts olfaction-mediated behaviors in Bactrocera dorsalis  

作　　者：Li Xu Hong-Bo Jiang Kai-Yue Tang Ying Yan Marc F.Schetelig Jin-Jun Wang 

机构地区：[1]Key Laboratory of Entomology and Pest Control Engineering,College of Plant Protection,Southwest University,Chongqing,China [2]International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control,Academy of Agricultural Sciences,Southwest University,Chongqing,China [3]Department of Insect Biotechnology in Plant Protection,Justus-Liebig-University Giessen,Institute for Insect Biotechnology,Giessen,Germany

出　　处：《Insect Science》2022年第5期1275-1286,共12页昆虫科学（英文版）

基　　金：supported by funding from the National Natural Science Foundation of China(31772233,32072491);Fok Ying Tung Education Foundation(161029);Chongqing Natural Science Foundation(cstc2019jcyj-zdxmX0034);Chongqing Young Talents Support Program and the China Agriculture Research System of MOF and MARA.

摘　　要：Olfaction plays an essential role in insect behavior such as host location,foraging,mating,and oviposition.The odorant receptor co-receptor(Orco)is an obligatory odorant receptor and indispensable in odor perception.Here,we characterized the Orco gene from the oriental fruit fly,Bactrocera dorsalis(Hendel),a notorious agriculture pest.The olfactory deficiency mutants were generated by editing the BdorOrco gene using the CRISPR/Cas9 system.Electroantennograms(EAG)and olfactory preference assays confirmed that BdorOrco^(−/−)mutant flies had reduced perception of methyl eugenol,β-caryophyllene,and ethyl acetate.Oviposition bioassays showed that the eggs laid by BdorOrco^(−/−)females mediated by benzothiazole and 1-octen-3-ol were significantly decreased.In addition,BdorOrco^(−/−)mutant flies took a significantly longer time to locate the food source compared with wild type(WT)flies.Altogether,our data indicated that Orco is essential for multiple physiological processes in B.dorsalis,and it expands our understanding of the function of insect Orco.

关 键 词：CRISPR/Cas9 FORAGING OLFACTION oriental fruit fly OVIPOSITION 

分 类 号：Q96[生物学—昆虫学]