Production of the yolk protein precursor vitellogenin is mediated by target of rapamycin (TOR) in the soft tick Ornithodoros moubata (Acari: Argasidae)  

作　　者：Meryem Behri Haruki Teshima Keisuke Kutsuwada Shoko Nakatake Mari H.Ogihara DeMar Taylor 

机构地区：[1]Graduate School of Life and Environmental Sciences,University of Tsukuba,Tsukuba,Ibaraki,Japan [2]Animal Breeding and Reproduction Research Division,Institute of Livestock and Grassland Science,National Agriculture and Food Research Organization,Tsukuba,Ibaraki,Japan

出　　处：《Insect Science》2022年第5期1299-1308,共10页昆虫科学（英文版）

基　　金：supported by Grants-in-Aid 16K08094 to DT from the Japan Society for the Promotion of Science(JSPS),Japan.

摘　　要：Initiation of vitellogenesis by blood feeding is essential for egg maturation in ticks.Nutrients derived from the blood meal are utilized by female ticks to synthesize the yolk protein precursor vitellogenin(Vg).Engorged Ornithodoros moubata ticks can synthesize Vg whether mated or virgin,thus O.moubata is an excellent model for studying the relative roles of blood feeding and mating in tick vitellogenesis.Injection of rapamycin into engorged O.moubata resulted in a reduction of ovarian growth and yolk accumulation in the oocytes of mated females.OmVg expression in the midgut and fat body and protein concentrations in the hemolymph significantly decreased in mated ticks after injection with rapamycin,indicating that inhibition of the nutrient-sensing target of rapamycin(TOR)pathway disrupts egg maturation at the levels of Vg expression and synthesis.These results suggest that the TOR-signaling pathway induces vitellogenesis in response to nutritional stimulation after a blood meal in O.moubata and is functionally independent of the mating-induced pathway.

关 键 词：Ornithodoros moubata target of rapamycin ticks vitellogenesis VITELLOGENIN 

分 类 号：Q96[生物学—昆虫学]