A reproducible and sensitive method for generating high-quality transcriptomes from single whitefly salivary glands and other low-input tissues  

作　　者：Marco Gebiola Brandon H.Le Kerry EMauck 

机构地区：[1]Department of Entomology,University of California Riverside,Riverside,California [2]Department of Botany and Plant Sciences,University of California Riverside,Riverside,California [3]Institute of Integrative Genome Biology,University of California Riverside,Riverside,California,USA

出　　处：《Insect Science》2022年第5期1318-1328,共11页昆虫科学（英文版）

基　　金：funded by USDA NIFA grant#2019-67014-29359 to K.M.;the National Institute of Health Training Grant in Environmental Toxicology T32 ES018827 to B.H.L.

摘　　要：Transcriptomic studies are an important tool for understanding the molecular pathways underlying host plant use by agricultural pests,including vectors of damaging plant pathogens.Thus far,bulk RNA-Seq has been the main approach for non-model insects.This method relies on pooling large numbers of whole organisms or hundreds of individually dissected organs.The latter approach is logistically challenging,may introduce artifacts of handling and storage,and is not compatible with biological replication.Here,we tested an approach to generate transcriptomes of individual salivary glands and other low-input body tissues from whiteflies(Bemisia tabaci MEAM1),which are major vectors of plant viruses.By comparing our outputs to published bulk RNA-Seq datasets for whole whitefly bodies and pools of salivary glands,we demonstrate that this approach recovers similar numbers of transcripts relative to bulk RNA-Seq in a tissue-specific manner,and for some metrics,exceeds performance of bulk tissue RNA-Seq.Libraries generated from individual salivary glands also yielded additional novel transcripts not identified in pooled salivary gland datasets,and had hundreds of enriched transcripts when compared with whole head tissues.Overall,our study demonstrates that it is feasible to produce high quality,replicated transcriptomes of whitefly salivary glands and other low-input tissues.We anticipate that our approach will expand hypothesis-driven research on salivary glands of whiteflies and other Hemiptera,thus enabling novel control strategies to disrupt feeding and virus transmission.

关 键 词：ALEYRODIDAE Bemisia tabaci bulk RNA-Seq insect vectors low-input RNA-Seq plant pathogens 

分 类 号：Q94[生物学—植物学]