Repeated exposure of fluazinam fungicides affects gene expression profiles yet carries no costs on a nontarget pest  

作　　者：Shahed Saifullah Aigi Margus Maaria Kankare Leena Lindström 

机构地区：[1]Department of Biological and Environmental Science,University of Jyväskylä,Jyväskylä,Finland

出　　处：《Insect Science》2022年第5期1373-1386,共14页昆虫科学（英文版）

基　　金：supported by the Finish Cultural foundation and the Academy of Finland(Grant NO.308302 to LL and 322980 to MK).

摘　　要：Fungicides are used to control pathogenic fungi of crop species,but they have also been shown to alter behavioral,life history and fitness related traits of nontarget insects.Here,we tested the fungicide effects on feeding behavior,survival and physiology of the nontarget pest insect,the Colorado potato beetle(CPB)(Leptinotarsa decemlineata).Feeding behavior was studied by a choice test of adult beetles,which were allowed to choose between a control and a fungicide(fluazinam)treated potato leaf.Larval survival was recorded after 24 and 72 h exposure to control and fungicide-treated leaves with 2 different concentrations.The adults did not show fungicide avoidance behavior.Similarly,survival of the larvae was not affected by the exposure to fungicides.Finally,to understand the effects of fungicides at the physiological level(gene expression),we tested whether the larval exposure to fungicide alter the expression of 5 metabolic pathway and stress associated genes.Highest concentration and 72-h exposure caused upregulation of 1 cytochrome P450(CYP9Z14v2)and 1 insecticide resistance gene(Ldace1),whereas metabolic detoxification gene(Ugt1)was downregulated.At 24-h exposure,highest concentration caused downregulation of another common detoxification gene(Gs),while both exposure times to lowest concentration caused upregulation of the Hsp70 stress tolerance gene.Despite these overall effects,there was a considerable amount of variation among different families in the gene expression levels.Even though the behavioral effects of the fungicide treatments were minor,the expression level differences of the studied genes indicate changes on the metabolic detoxifications and stress-related pathways.

关 键 词：behavior FLUAZINAM gene expression Leptinotarsa decemlineata metabolic detoxification nontarget animal 

分 类 号：Q96[生物学—昆虫学]