The actions of neonicotinoid insecticides on nicotinic acetylcholine subunits Ldα1 and Ldα8 of Leptinotarsa decemlineata and assembled receptors  

作　　者：Zhi-Min Wang Sha Li Cheng-Cheng Shi Lin-Jie Xie Kai-Yun Fu Wei-Hua Jiang 

机构地区：[1]College of Plant Protection,Nanjing Agricultural University/Key Laboratory of Integrated Management of Crop Disease and Pests,Ministry of Education/Key Laboratory of Integrated Pest Management on Crops in East China,Ministry of Agriculture,Nanjing 210095,China [2]Institute of Plant Protection,Xinjiang Academy of Agricultural Sciences/Key Laboratory of Integrated Pest Management on Crop in Northwestern Oasis,Ministry Agriculture P.R.China,Urumqi,China

出　　处：《Insect Science》2022年第5期1387-1400,共14页昆虫科学（英文版）

基　　金：funded by National Key Re-search and Development Project of China(No.2018YFD0200802).

摘　　要：The insect nicotinic acetylcholine receptor(nAChR)is a pentameric channel protein and also a target for neonicotinoids.There are few reported studies on the molecular interactions of Leptinotarsa decemlineata nAChRs with neonicotinoids.In this study,we analyzed the response of acetylcholine and neonicotinoids(thiamethoxam[TMX],imidacloprid[IMI],and clothianidin[CLO])on hybrid receptors constructed by nAChRα1 andα8 subunits of L.decemlineata(Ldα1 and Ldα8)co-expressed with ratβ2 subunit(rβ2)at different capped RNA(cRNA)ratios in Xenopus oocytes.In addition,we evaluated the expression changes of Ldα1 and Ldα8 after median lethal dose of TMX treatment for 72 h by quantitative polymerase chain reaction(qPCR).The resulting functional nAChRs Ldα1/rβ2 and Ldα1/Ldα8/rβ2 showed different pharmacological characteristics.The neonicotinoids tested showed lower agonist affinity on Ldα1/Ldα8/rβ2 compared to Ldα1/rβ2 at same ratios of subunit cRNAs.The sensitivities of neonicotinoids tested for Ldα1/rβ2 and Ldα1/Ldα8/rβ2 at cRNA ratios of 5:1,1:1 and 5:5:1,1:1:1,respectively,were lower than those for nAChRs at ratios of 1:5 and 1:1:5,respectively,whereas the values of maximum response(Imax)varied.For Ldα1/Ldα8/rβ2,a reduction of Lda8 cRNA resulted in increased sensitivity to IMI and decreased sensitivity to TMX.The expression of Ldα1 and Ldα8 significantly decreased in adults by 82.12%and 47.02%,respectively,while Ldα8 was significantly upregulated by 2.44 times in 4th instar larvae after exposure to TMX.We infer that Ldα1 and Ldα8 together play an important role in the sensitivity of L.decemlineata to neonicotinoids.

关 键 词：expression IMIDACLOPRID Leptinotarsa decemlineata nicotinic acetylcholine receptor THIAMETHOXAM Xenopus oocyte 

分 类 号：S48[农业科学—农药学]