基于金纳米线构建电化学传感器间接检测碱性磷酸酶活性  

Indirect detection of alkaline phosphatase activity by electrochemical sensor based on gold nanowires

作　　者：余婷婷 王敏 文颖 杨海峰 YU Tingting;WANG Min;WEN Ying;YANG Haifeng(College of Chemistry and Materials Science,Shanghai Normal University,Shanghai 200234,China)

机构地区：[1]上海师范大学化学与材料科学学院,上海200234

出　　处：《上海师范大学学报（自然科学版）》2022年第5期582-589,共8页Journal of Shanghai Normal University(Natural Sciences)

基　　金：国家自然科学基金(21475088)。

摘　　要：在临床实践中,碱性磷酸酶(ALP)是最常检测的酶之一,已被广泛用作肝病、骨病、乳腺癌、前列腺癌以及糖尿病等多种疾病的重要检测指标.通过自组装方法在氧化铟锡(ITO)导电玻璃上制备金纳米线(AuNWs),利用4-巯基苯甲酸(4-MBA)修饰AuNWs,对其进行改性,与铜离子(Cu^(2+))螯合形成Cu^(2+)-AuNWs/ITO电极.在ALP活性检测中,电解液中的焦磷酸根阴离子(PPi)与电极表面Cu^(2+)结合,湮灭了Cu^(2+)的电化学信号.ALP的加入使底物PPi水解并释放出Cu^(2+),电化学信号增加.构建的Cu^(2+)-AuNWs/ITO电极对ALP活性检测范围为60~180 U·L^(-1),且抗干扰性能良好.In the clinical practice,alkaline phosphatase(ALP)is one of the most frequently detected enzymes.It has been used as an important indicator of liver disease,bone disease,breast cancer,prostate cancer,diabetes,and other diseases.In this report,gold nanowires(AuNWs)were firstly prepared on indium tin oxide(ITO)conductive glass by self-assembly method.Then,AuNWs were modified by 4-mercaptobenzoic acid(4-MBA)and chelated with copper ions(Cu^(2+))to form the Cu^(2+)-AuNWs/ITO electrode.In the detection of ALP activity,pyrophosphate anion(PPi)in the electrolyte combines with copper ions on the electrode surface to annihilate the electrochemical signal of copper ions.However,the addition of ALP hydrolyzes the substrate PPi and releases copper ions,which increases the electrochemical signal.The as-constructed Cu^(2+)-AuNWs/ITO electrode can detect ALP activity in the range of 60~180 U·L^(-1) with the high anti-interference performance.

关 键 词：碱性磷酸酶(ALP) 金纳米线(AuNWs) 无标记检测 电化学传感器 

分 类 号：O647.3[理学—物理化学]