基于WGCNA鉴定山羊金黄色葡萄球菌型乳腺炎关键应答基因  

Identification of Key Response Genes for S.aureus-type Mastitis in Goats by WGCNA

作　　者：王丽敏 靳丽娜 刘亚芳 闫晓茹 郑惠玲[1] WANG Limin;JIN Lina;LIU Yafang;YAN Xiaoru;ZHENG Huiling(College of Animal Science and Technology,Northwest A&F University,Yangling,712100,China)

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《家畜生态学报》2022年第10期71-77,共7页Journal of Domestic Animal Ecology

基　　金：陕西省重点研发计划项目(2022ZDLNY01-02);陕西省自然基金重点项目(2022JZ-10);西北农林科技大学研究生教育教学改革研究项目(JXGG2015)。

摘　　要：该研究旨在通过加权基因共表达网络分析(weighted gene co-expression network analysis,WGCNA)筛选金黄色葡萄球菌感染山羊乳腺的关键应答基因。从GEO数据库下载乳腺感染金黄色葡萄球菌24 h山羊乳汁中体细胞的转录组数据集GSE33894,通过WGCNA、基因本体(gene ontology,GO)富集分析、蛋白互作(protein-protein interaction,PPI)网络等方法,得到与山羊乳腺感染金黄色葡萄球菌24 h相关的2个目标模块及每个模块的30个枢纽基因,同时得到目标模块的PPI网络及每个网络的30个核心基因。最终取交集共得到4个山羊乳腺感染金黄色葡萄球菌24 h的关键应答基因(NFKB1、MRPL16、TMED10、ERLEC1)。研究结果为进一步揭示金黄色葡萄球菌型乳腺炎的发生发展分子机制提供了新的参考。The purpose of this study was to select the key response genes of S.aureus infection by weighted gene co-expression network analysis(WGCNA).In this study,the transcriptome dataset GSE33894 of breast-infected S.aureus 24 h in goats was downloaded from the GEO database.By WGCNA,GO(Gene Ontology)enrichment analysis,protein-protein interaction(PPI)network and other methods,two target modules and 30 hub genes per module associated with goat mammary S.aureus infection for 24 h were obtained,the PPI network of the target module and the 30 core genes of each network were also obtained.Finally,the intersection yielded four key response genes(NFKB1,MRPL16,TMED10,ERLEC1)of goat breast infected with S.aureus for 24 h.The results provide a new reference to further reveal the molecular mechanism of the development of S.aureus-type mastitis.

关 键 词：乳腺炎 金黄色葡萄球菌 应答基因 加权基因共表达网络分析 

分 类 号：S852.61[农业科学—基础兽医学] S858.27[农业科学—兽医学]