惯性力辅助微流控芯片-ICPMS研究单细胞对纳米Al_(2)O_(3)的摄取  

Inertial force-assisted microfluidic chip-ICPMS for the study of nano-Al_(2)O_(3)uptake by single cells

作　　者：魏星 宋扬 魏榆珈 陈明丽[1] 王建华 Xing Wei;Yang Song;Yu-Jia Wei;Ming-Li Chen;Jian-Hua Wang(Research Center for Analytical Sciences,Department of Chemistry,College of Sciences,Northeastern University,Shenyang 110819,China)

机构地区：[1]东北大学理学院化学系分析科学研究中心,沈阳110819

出　　处：《中国科学：化学》2022年第9期1730-1736,共7页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金(编号:21727811,22104012,22074011);中央高校基本科研业务费(编号:N2005003,N2105017,N2205027)资助项目。

摘　　要：随着人类对生命体系研究的深入,阐明细胞异质性的单细胞分析已成为生命分析的研究热点.基于流体动力学原理的惯性微流控技术在单细胞聚焦分离中表现出优越的性能.为深入探究螺旋结构对单细胞聚焦的影响,我们设计了三种不同半径变化的螺旋微流控芯片用于单细胞聚焦分离,结合电感耦合等离子体质谱(ICP-MS)构建了单细胞分析系统,研究了A549细胞对纳米Al_(2)O_(3)(nano-Al_(2)O_(3))的摄取行为.研究表明,半径每90°变化的螺旋型芯片在通道内可生成更强的Dean涡流,更有利于单细胞聚焦.经nano-Al_(2)O_(3)孵育后,细胞间铝含量分布存在明显差异,在单细胞层面证明A549细胞对nano-Al_(2)O_(3)的摄取存在异质性.本研究设计的芯片可在较短通道及较低流量下实现单细胞有效聚焦,为单细胞分析提供了新的平台.As the heterogeneity between cells is gradually recognized,single-cell analysis has become a hot topic in the field of life science analysis.Inertial microfluidics based on fluid dynamics have shown superior performance in the field of single cell focusing and separation,and have received widespread attention.In the present study,three spiral microfluidic chip configurations with different radius variations are designed and coupled with inductively coupled plasma mass spectrometry(ICP-MS)to construct a single-cell analysis system to study the uptake of nano-Al_(2)O_(3)by A549 cells.The results show that the spiral chip with every 90°radius variation could generate a stronger Dean flow in the microchannel,which is more favorable for cell focusing.The heterogeneity of nano-Al_(2)O_(3)uptake by A549 cells is demonstrated at the single cell level by the significant difference in aluminum content between individual cells after incubation.The chip designed in this study can effectively focus cells at lower volumetric flow rates and shorter channel lengths,providing a new platform for single-cell analysis.

关 键 词：单细胞分析 惯性微流控 电感耦合等离子体质谱 nano-Al_(2)O_(3) 

分 类 号：Q2-33[生物学—细胞生物学] O657.63[理学—分析化学]