大白菜黄子叶突变体的遗传分析及其基因定位  

Genetic Analysis and Gene Mapping of a Yellow Cotyledon Mutant in Chinese Cabbage(Brassica rapa ssp.pekinensis)

作　　者：张妮南 薛一花 吴俊清[1] 和禹廷 王亚秀 张学芬 刘小愿 张鲁刚[1] ZHANG Ni-Nan;XUE Yi-Hua;WU Jun-Qing;HE Yu-Ting;WANG Ya-Xiu;ZHANG Xue-Fen;LIU Xiao-Yuan;ZHANG Lu-Gang(College of Horticulture,Northwest A&F University,Yangling 712100,China)

机构地区：[1]西北农林科技大学园艺学院,杨凌712100

出　　处：《农业生物技术学报》2022年第11期2077-2085,共9页Journal of Agricultural Biotechnology

基　　金：国家重点研发计划(2016YFD0101701;2017YFD0101802);杨凌种业创新中心重点研发计划(YIzy-sc-04)。

摘　　要：大白菜(Brassica rapa ssp.pekinensis)原产于中国,属于十字花科芸薹属二年生草本植物,是我国栽培面积最大的一种蔬菜作物。本研究以课题组前期在普通大白菜与紫心大白菜杂交后代中发现的黄子叶突变体,经自交分离获得了遗传稳定的黄子叶纯系(19YC-2)为研究对象,与正常绿子叶近等基因系(19GC-2)杂交构建F_(1)和F_(2)群体为实验材料,对大白菜黄子叶突变体进行遗传分析及基因定位。表型观测表明,黄子叶纯系与正常绿子叶近等基因系杂交的F_(1)植株子叶表型均为正常绿色,F_(2)群体中绿子叶单株数比黄子叶单株数符合3∶1分离规律,说明黄子叶突变是由单隐性核基因控制的质量性状。进一步用混合分离群(分组)分析法(bulked segregant analysis,BSA)筛选双亲多态性SSR引物,将黄子叶基因定位在大白菜A06连锁群上,通过在定位区间加密设计SSR引物,将黄子叶基因(Brassica rapa yellow cotyledon,Bryc)定位于SSR280-23和SSR280-39两个标记之间,遗传距离分别为0.04和0.96 cM,物理距离为83.9 kb。本研究通过对大白菜黄子叶突变体进行遗传分析和基因定位,为黄子叶基因Bryc的克隆及其分子机理研究奠定基础。Chinese cabbage(Brassica rapa ssp.pekinensis)is native to China and belongs to the crucifer family Brassica genus biennial plant.It is a vegetable crop widely cultivated in our country.In this study,a genetically stable yellow cotyledon pure line(19YC-2)and normal green cotyledon near isogenic line(19GC-2)and the F_(1),F_(2)populations by hybridizing with 19YC-2 and 19GC-2 were used as materials,The genetic analysis and gene mapping of yellow cotyledon mutant in Chinese cabbage were carried out.Phenotypic observation showed that the cotyledon phenotype of F_(1) plants was normal green as 19GC-2,and the number of green cotyledon plants to yellow cotyledon plants in F_(2)population was conformed to 3∶1 proportion,which indicated that yellow cotyledon mutation was a quality trait controlled by one single recessive nuclear gene.The parental polymorphic SSR primers were further screened by bulked segregant analysis(BSA)method and the gene mapping of yellow cotyledon was made.The yellow cotyledon gene was located on the A06 linkage group of Chinese cabbage with two nearest flanking markers SSR280-23 and SSR280-42,their genetic distances are 0.04 cM and 0.96 cM,respectively,and their interval physical distance was 83.9 kb.Through genetic analysis and gene mapping of the yellow cotyledon mutants of Chinese cabbage,this study lays a foundation for the cloning and molecular mechanism of Brassica rapa yellow cotyledon(Bryc).

关 键 词：大白菜 黄子叶突变体 遗传分析 基因定位 

分 类 号：S634.1[农业科学—蔬菜学]