根际促生菌S1菌株的分离鉴定及促生效果  

Isolation and Identification of Plant Growth Promoting Rhizobacteria S1 and Determination of Its Effect on Growth Promotion

作　　者：吕朝阳 王威 杨淼泠 施李鸣[1] 张维 张克诚[1] 葛蓓孛[1] LU Zhao-yang;WANG Wei;YANG Miao-ling(Institute of Plant Protection,Chinese Academy of Agricultural Science,Beijing 100193)

机构地区：[1]中国农业科学院植物保护研究所,北京100193

出　　处：《安徽农业科学》2022年第20期81-85,107,共6页Journal of Anhui Agricultural Sciences

基　　金：广西科技重大专项(桂科AA18242026)。

摘　　要：[目的]筛选出更多新型、高效的植物根际促生菌,研发绿色环保的微生物肥料及植物生长调节剂。[方法]从广西北海互花米草根际采集土壤样品,采用稀释平板法分离土壤样品中的微生物。通过检测菌株分泌IAA和溶磷的能力,从分离的产物中筛选出解磷能力强及IAA高产菌株,并制备菌株发酵液对番茄种子和幼苗分别进行浸种和灌根,培养到适宜时间后测定种子和幼苗的各生长指标。[结果]发酵液浓度为1×10^(4) CFU/mL时能够显著促进番茄种子萌发和幼苗生长。经16S rRNA及生理生化鉴定,该菌株为阴沟肠杆菌(Enterobacter cloacae)。[结论]筛选出1株具有溶磷、分泌生长素性能的根际优良促生菌株——阴沟肠杆菌(Enterobacter cloacae),该菌株对番茄种子的萌发及幼苗的生长有显著的促进作用。[Objective]In order to screen out more new and efficient plant rhizosphere growth-promoting bacteria,and to develop green and environmental friendly microbial fertilizer and plant growth regulator.[Method]Soil samples were collected from the rhizosphere of Spartina alterniflora in Beihai,Guangxi.Microorganisms were isolated by dilution plate method.By detecting the ability of the strains to secrete IAA and solubilizing phosphorus,the strains with strong phosphorus solubilizing ability and high IAA yield were selected from these strains,and then the strain fermentation broth was prepared to soak and irrigate tomato seeds and seedlings.After the cultivation to the appropriate time,the growth indexes of the seeds and seedlings were measured.[Result]The results showed that when the concentration of the fermentation broth was 1×10^(4) CFU/mL,it could significantly promote tomato seed germination and seedling growth.The strain was identified as Enterobacter cloacae by 16S rRNA.[Conclusion]A strain of Enterobacter cloacae,an excellent rhizosphere growth-promoting strain with the properties of dissolving phosphorus and secreting auxin,was screened out.The strain had a significant promoting effect on the germination of tomato seeds and seedling growth.

关 键 词：植物根际促生菌 阴沟肠杆菌 微生物肥料 番茄 

分 类 号：S154.3[农业科学—土壤学]