植物源农药银杏酚酸提取工艺研究  

Study on the Extraction Process of Ginkgo Phenolic Acid from Botanical Pesticide

作　　者：张翠翠 于蓓蓓[1] 闫雪生[1] 王芳[2] 林慧彬[1] ZHANG Cui-cui;YU Bei-bei;YAN Xue-sheng(Shandong Academy of Traditional Chinese Medicine,Jinan,Shandong 250014)

机构地区：[1]山东省中医药研究院,山东济南250014 [2]山东中医药大学附属医院,山东济南250013

出　　处：《安徽农业科学》2022年第20期151-154,共4页Journal of Anhui Agricultural Sciences

基　　金：国家重点研发计划(2017YFC1701500,2017YFC1701502,2017YFC1701504,2017YFC1702701);中央本级重大增减支项目(2060302);济南市农业应用技术创新计划项目(CX202112)。

摘　　要：[目的]利用正交设计优化从银杏外种皮中提取银杏酚酸的工艺条件,为植物源农药的开发奠定基础。[方法]采用二氯甲烷回流提取方法,以银杏酚酸的提取量为指标,采用正交试验设计的方法,系统考察提取时间、料液比、提取温度和提取次数4个因素对银杏酚酸提取工艺的影响,用高效液相色谱法测定银杏酚酸的含量。[结果]以二氯甲烷为提取溶剂,采用1∶10(g∶mL)的料液比,在60℃条件下回流提取2次,每次1 h为最优提取条件;相关因素方差分析表明,提取温度对提取效果有显著影响。[结论]该提取工艺稳定可靠、成本低,具有应用价值。[Objective]Orthogonal design was used to optimize the technological conditions for extracting ginkgolic acids from ginkgo seed coats,laying a foundation for the development of plant-derived pesticides.[Method]The extraction amount of ginkgo phenolic acid was measured by dichloromethane reflux method,and the method of orthogonal experimental design was used to systematically extract.The effects of extraction time,solid-liquid ratio,extraction temperature and extraction times on the extraction process of ginkgo phenolic acid were determined by high performance liquid chromatography(HPLC).[Result]Using dichloromethane as extraction solvent,using 1∶10(g∶mL)solid-liquid ratio,refluxing at 60℃for 2 times,each time for 1 h was the optimal extraction condition.Correlation factor analysis showed that the extraction temperature had a significant effect on the extraction effect.[Conclusion]The extraction process is stable and reliable,low cost and has application value.

关 键 词：银杏酚酸 植物源农药 回流提取 工艺优化 正交试验 

分 类 号：R284.2[医药卫生—中药学]