LED多波段光谱治疗对大鼠皮肤创伤愈合的影响  

Effect of LED Multiband Spectral Therapy on Skin Wound Healing in Rats

作　　者：柏鑫 侯雪峰 何文芳 何玥颖 王中琪 吴西 陈克明 BAI Xin;HOU Xue-feng;HE Wen-fang;HE Yue-ying;WANG Zhong-qi;WU Xi;CHEN Ke-ming(Clinical College of Traditional Chinese Medicine,Gansu University of Chinese Medicine,Lanzhou 730030,Gansu,China;Basic Medical Laboratory,the 940th Hospital of Joint Logistic Support Force of PLA,Lanzhou 730050,Gansu,China)

机构地区：[1]甘肃中医药大学中医临床学院,甘肃兰州730030 [2]解放军联勤保障部队第九四〇医院基础医学实验室,甘肃兰州730050

出　　处：《医学信息》2022年第18期34-38,共5页Journal of Medical Information

基　　金：军队后勤科研计划项目(编号:CLB18J028)。

摘　　要：目的研究LED多波段光谱治疗对大鼠皮肤创伤愈合的影响。方法选取50只雄性Wistar大鼠,在大鼠背部制备小面积全层皮肤缺损创面模型,随机分为模型对照(MC)组、LED治疗(LEDT)组、红外线治疗(IR)组、红光治疗(RL)组以及紫外线治疗(UV)组,每组10只。各治疗组大鼠每天暴露于对应光源下照射40 min,模型对照组放入治疗仪但不通电。每组分别于第3、7、14天处死3只大鼠,计算创面愈合率,观察HE染色结果,并检测相关蛋白表达量。结果LEDT组、IR组、RL组和UV组创面愈合率治疗第3、7、14天愈合率均高于MC组(P<0.05);创面HE染色显示,LED多波段光谱治疗能够降低炎症反应,促进皮肤组织增殖分化,加速创伤愈合进程;LEDT组、IR组、RL组和UV组治疗第14天后EGF、VEGF、TGF-β1表达量均高于MC组(P<0.05),但LEDT与IR组TGF-β1比较,差异无统计学意义(P>0.05)。结论LED多波段光谱治疗能够促进大鼠皮肤创伤愈合,促进皮肤再上皮化进程,提高创面中EGF、TGF-β1及VEGF蛋白表达水平。Objective To study the effect of LED multi-band spectral therapy on skin wound healing in rats.Methods Fifty male Wistar rats were randomly divided into model control(MC)group,LED treatment(LEDT)group,infrared treatment(IR)group,red light treatment(RL)group and ultraviolet treatment(UV)group,with 10 rats in each group.The rats in each treatment group were exposed to the corresponding light source for 40 minutes every day,and the model control group was put into the therapeutic apparatus but not electrified.Three rats in each group were sacrificed on the 3 rd,7 th and 14 th day respectively.The wound healing rate was calculated,the results of HE staining were observed,and the expression of related proteins was detected.Results The wound healing rate of LEDT group,IR group,RL group and UV group was higher than that of MC group on the 3 rd,7 th and 14 th day of treatment(P<0.05).Wound HE staining showed that LED multi-band spectroscopy could reduce the inflammatory response,promote the proliferation and differentiation of skin tissue,and accelerate the process of wound healing.The expression levels of EGF,VEGF and TGF-β1 in LEDT group,IR group,RL group and UV group were higher than those in MC group after 14 days of treatment(P<0.05),but there was no significant difference in TGF-β1 between LEDT group and IR group(P>0.05).Conclusion LED multi-band spectral therapy can promote the healing of skin wounds in rats,promote the process of skin re-epithelialization,and increase the expression levels of EGF,TGF-β1 and VEGF proteins in wounds.

关 键 词：LED多波段光谱治疗 创面愈合 红外线 红光 紫外线 

分 类 号：R44[医药卫生—诊断学]